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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
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- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553729, 557307, or 561828), APC Rat Anti-Mouse CD25 (Cat. No. 557192 or 561048), and PE Rat IgG2b, κ Isotype Control (Cat. No. 555848) or PE Rat Anti-Mouse CD39 antibody (Cat. No. 567104) at 0.5 μg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. Upper panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 (right) or Ig Isotype control (left) versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) lymphocytes. Lower panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 (right) or Ig Isotype control (left) versus CD25 staining were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Rat Anti-Mouse CD39
BD Pharmingen™ PE Rat Anti-Mouse CD39
BD Pharmingen™ PE Rat Anti-Mouse CD39
BD Pharmingen™ PE Rat Anti-Mouse CD39
BD Pharmingen™ PE Rat Anti-Mouse CD39
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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
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- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
配套商品
The Y23-1185 monoclonal antibody specifically recognizes mouse CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), which is an enzyme on the surface of vascular endothelial cells, antigen presenting cells and activated immune cells. CD39 is encoded by ENTPD1 which belongs to the ectoenzyme family. The catalytic portion of the CD39 is extracellular, where it acts on extracellular nucleoside triphosphates and diphosphates, including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.
研发参考 (5)
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Allard D, Allard B, Stagg J. On the mechanism of anti-CD39 immune checkpoint therapy.. J Immunother Cancer. 2020; 8(1):e000186. (Biology). 查看参考
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Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology). 查看参考
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Mizumoto N, Kumamoto T, Robson SC, et al. CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness.. Nat Med. 2002; 8(4):358-365. (Biology). 查看参考
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Salmi M, Jalkanen S. Ectoenzymes controlling leukocyte traffic.. Eur J Immunol. 2012; 42(2):284-92. (Biology). 查看参考
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Zhou Q, Yan J, Putheti P, et al. 2009; 9(10):2303-2311. (Biology). 查看参考
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