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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Multicolor flow cytometric analysis of adult mouse spleen cells and bone marrow hematopoietic stem cells. (Panel A) BALB/c spleen cells were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092) and PE Rat Anti-Mouse CD150 (Cat. No. 562651), staining cells well above background compared to PE Rat IgG2a, κ isotype control (Cat. No. 557690) (data not shown). A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by events with the forward and side-light scattering characteristics of viable lymphocytes. (Panel B) BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE-Cy™7 Hamster Anti-Mouse CD48 (Cat. No. 560731), and PE Rat Anti-Mouse CD150 antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P4 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated). Lineage-negative and CD48- CD150+ (Middle Plot, P3 Gate) bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ PE Rat anti-Mouse CD150
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准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The Q38-480 monoclonal antibody specifically binds to mouse CD150, also known as SLAM (signaling lymphocyte activation molecule). CD150 is a type 1 transmembrane glycoprotein that is a member of the CD2 subfamily of the Ig superfamily. It is encoded by the Slamf1 (signaling lymphocytic activation molecule family member 1) gene. CD150 is differentially expressed on subsets of thymocytes, T and B lymphocytes, dendritic cells, macrophages, and endothelial cells. SLAM plays multiple roles in innate and adaptive immunity serving as an adhesion molecule and/or coreceptor. CD150-mediated costimulation of TCR-activated T cells reportedly results in the increased production of IFN-γ by Th1 cells and is required for IL-4 production by T follicular helper cells. CD150 also plays important roles in hematopoietic cell developmental pathways. CD150 is differentially expressed by self-renewing adult hematopoietic stem cells (HSC) whereas non-multipotent hematopoietic progenitor cells are CD150-. Utilizing additional cell surface markers, lineage-negative CD150+CD48-CD41- cell fractions are reported to be highly enriched for adult HSC.
研发参考 (4)
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Castro AG, Hauser TM, Cocks BG, et al. Molecular and functional characterization of mouse signaling lymphocytic activation molecule (SLAM): differential expression and responsiveness in Th1 and Th2 cells.. J Immunol. 1999; 163(11):5860-70. (Biology). 查看参考
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Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005; 121(7):1109-1121. (Biology). 查看参考
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Wang N, Satoskar A, Faubion W, et al. The cell surface receptor SLAM controls T cell and macrophage functions. J Exp Med. 2004; 199(9):1255-1264. (Biology). 查看参考
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Yusuf I, Kageyama R, Monticelli L, et al. Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150). J Immunol. 2010; 185(1):190-202. (Biology). 查看参考
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