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PE Rat anti-Human TLR9
PE Rat anti-Human TLR9

Flow cytometric analysis of PE anti-human TLR9 on resting human peripheral blood mononuclear cells (PBMC). Human PBMC were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714) followed by staining with PE anti-human TLR9, PerCP-Cy5.5 anti-human HLA-DR (Cat. No. 339194) and APC Mouse anti-Human CD123 (left image, clone 7G3, Cat. No. 560087) or FITC anti-human CD11c (right image, clone B-ly6). The dot plots were derived from a HLA-DR+ gate. Flow cytometry was performed on a BD FACSCalibur™ System.

Flow cytometric analysis of PE anti-human TLR9 on resting human peripheral blood mononuclear cells (PBMC). Human PBMC were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714) followed by staining with PE anti-human TLR9, PerCP-Cy5.5 anti-human HLA-DR (Cat. No. 339194) and APC Mouse anti-Human CD123 (left image, clone 7G3, Cat. No. 560087) or FITC anti-human CD11c (right image, clone B-ly6). The dot plots were derived from a HLA-DR+ gate. Flow cytometry was performed on a BD FACSCalibur™ System.

商品详情
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BD Pharmingen™
CD289, toll-like receptor 9
Human (QC Testing)
Rat IgG2a, κ
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645543
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
560425 Rev. 1
抗体详情
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eB72-1665

Toll-like receptor 9 (TLR9), also designated as CD289, is a type 1 transmembrane glycoprotein (~110-119 kDa calculated molecular weights for protein isoforms) encoded by the TLR9 gene in chromosome 3. TLR9 is a member of the Toll-like receptor (TLR) family whose members play fundamental roles in pathogen recognition and activation of innate immunity. TLR9 is highly expressed by plasmacytoid dendritic cells and B cells and at lower levels in some other cell types. TLR9 is expressed predominantly in the endoplasmic reticulum. It is recruited to endosomal and lysosomal compartments upon cellular stimulation by bacterial, viral or synthetic DNA molecules that contain unmethylated CpG dinucleotide sequences. TLR9 generated signals lead to the activation of genes that express type-I interferons and proinflammatory cytokines including IL-6 and IL-12. TLR9-mediated signaling is also implicated in acquired immune responses as well as in the pathogenesis of autoimmune diseases. The eB72-1665.1 mAb recognizes the human TLR9 epitope from the peptide sequence, amino acids 273-288.

560425 Rev. 1
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560425 Rev.1
报价单和参考
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研发参考 (6)

  1. Akira S, Takeda K. Toll-like receptor signalling. Nat Rev Immunol. 2004; 4(7):499-511. (Biology). 查看参考
  2. Chuang TH, Ulevitch RJ. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw. 2000; 11(3):372-378. (Immunogen). 查看参考
  3. Hemmi H, Takeuchi O, Kawai T, et al. A Toll-like receptor recognizes bacterial DNA. Nature. 2000; 408(6813):740-745. (Biology). 查看参考
  4. Kumagai Y, Takeuchi O, Akira S. TLR9 as a key receptor for the recognition of DNA. Adv Drug Deliv Rev. 2008; 60(7):795-804. (Biology). 查看参考
  5. Latz E, Schoenemeyer A, Visintin A, et al. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 2004; 5(2):190-198. (Biology). 查看参考
  6. Leifer CA, Kennedy MN, Mazzoni A, Lee C, Kruhlak MJ, Segal DM. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol. 2004; 173(2):1179-1183. (Biology). 查看参考
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560425 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.