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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Multicolor flow cytometric analyses of XBP-1S expressed in human and mouse cells. Panel 1. Analysis of XBP-1S expressed in activated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with 10 μg/ml ODN 2006 Type B CpG Oligonucleotide (Invivogen, Cat. No. tlrl-2006-1) at 37˚C for 7 days. The cells were harvested and incubated with BD Horizon™ Fixable Viability Stain 450 (Cat. No. 562247), fixed in 1X Fix/Perm Buffer (BD Pharmingen™ Transcription Buffer Set, Cat: 562574) at 2-8˚C (45 min) and permeabilized in 1X Perm/Wash Buffer (Transcription Buffer Set) at 2-8˚C (45 min). Cells were stained with PE Mouse anti-XBP-1S (Cat. No. 562642) and PerCP-Cy™5.5 Mouse Anti-Human CD20 (Cat. No. 558021) antibodies. Two-color flow cytometric contour plots showing the correlated expression of CD20 versus XBP-1S (or Ig isotype control staining) were derived from live-gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System. Panel 2. Analysis of XBP-1S expressed in activated mouse splenocytes. BALB/C splenocytes were stimulated with 1 μg/ml lipopolysaccharide (LPS; Sigma Cat. No. L3137) at 37˚C (3 days). Cells were incubated with Fixable Viability Stain 450, fixed in 1X Fix/Perm Buffer, and permeabilized in 1X Perm/Wash Buffer as described for Panel 1. The cells were stained with PE Mouse anti-XBP-1S and APC Rat Anti-Mouse CD45R/B220 (BD Cat. No. 553092). The cells were then analyzed as described above. Contour plots showing the correlated expression of B220 versus XBP-1S (or Ig isotype control staining) were derived from live-gated events with the forward and side light-scatter characteristics of intact lymphocytes.
BD Pharmingen™ PE Mouse anti-XBP-1S
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Note: For optimal immunofluorescent staining of XBP1-S, the Transcription Factor Buffer Set (Cat. No. 562574) is highly recommended for the brightest staining results.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
配套商品
The Q3-695 monoclonal antibody specifically binds to XBP-1S, the spliced isoform of X-box binding protein 1 (XBP-1). XBP-1S is generated by cells that undergo an unfolded protein response, ie, cells responding to the accumulation of unfolded proteins in the endoplasmic reticulum. It plays important roles as a transcription factor in a number of important cellular processes including the regulation of MHC class II genes, differentiation of plasma cells, immunoglobulin secretion and hepatocyte growth.
研发参考 (3)
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Gass JN, Gifford NM, Brewer JW. Activation of an unfolded protein response during differentiation of antibody-secreting B cells. J Biol Chem. 2002; 277(50):49047-49054. (Biology). 查看参考
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Iwakoshi NN, Lee AH, Vallabhajosyula P, Otipoby KL, Rajewsky K, Glimcher LH. Plasma cell differentiation and the unfolded protein response intersect at the transcription factor XBP-1. Nat Immunol. 2003; 4(4):321-329. (Biology). 查看参考
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Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001; 107(7):881-891. (Biology). 查看参考
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