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Expression of Notch1 in double-negative thymocytes. Fixed and permeabilized C57BL/6 thymocytes were stained with either PE Mouse anti-Mouse Notch1 or PE Mouse IgG1, κ Isotype Control MOPC-31C (Cat. No. 550617, open histogram). Cells were then stained with FITC Rat anti-Mouse CD4 (Cat. No. 553046/553047) and APC Rat anti-Mouse CD8a (Cat. No. 553035). For analysis of Notch1 expression, an electronic gate was set to include only the CD4-CD8a subpopulation. Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
BD Pharmingen™ PE Mouse Anti-Mouse Notch1
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The Notch family of transmembrane receptors controls cell-fate "decisions" during the development of many organs in a wide variety of animal species. After binding its ligand, a Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with mouse Notch2, 3, or 4. In the mouse, Notch1 mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. Using mAb mN1A, Notch1 is detected in CD4-CD8- (double-negative) and CD4-CD8+ thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis. The mN1A mAb does not cross-react with rat thymocytes. An alternative anti-mouse Notch1 monoclonal antibody, clone 22E5.5, specifically binds to an extracellular domain of mouse Notch1.
研发参考 (8)
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Anderson AC, Robey EA, Huang YH. Notch signaling in lymphocyte development. Curr Opin Genet Dev. 2001; 11(5):554-560. (Biology). 查看参考
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Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development.. Science. 1999; 284(5415):770-6. (Biology). 查看参考
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Huppert SS, Le A, Schroeter EH, et al. Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. Nature. 2000; 405(6789):966-970. (Immunogen). 查看参考
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Kawamata S, Du C, Li K, Lavau C. Notch1 perturbation of hemopoiesis involves non-cell- autonomous modifications. J Immunol. 2002; 168(4):1738-1745. (Biology). 查看参考
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Kojika S, Griffin JD. Notch receptors and hematopoiesis. Exp Hematol. 2001; 29(9):1041-1052. (Biology). 查看参考
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Milner LA, Bigas A, Kopan R, Brashem-Stein C, Bernstein ID, Martin DI. Inhibition of granulocytic differentiation by mNotch1. Proc Natl Acad Sci U S A. 1996; 93(23):13014-13019. (Biology). 查看参考
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Milner LA, Bigas A. Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation. Blood. 1999; 93(8):2431-2448. (Biology). 查看参考
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Walker L, Carlson A, Tan-Pertel HT, Weinmaster G, Gasson J. The notch receptor and its ligands are selectively expressed during hematopoietic development in the mouse. Stem Cells. 2001; 19(6):543-552. (Biology). 查看参考
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