商品详情
BD Pharmingen™
Th-POK; THPOK; c-Krox; hcKrox
Human (QC Testing)
Mouse IgG1, κ
Human cKrox aa. 213-337
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl/test
AB_2869707
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 ML
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 ML
品类编号
554657
Transcription Factor Buffer Set RUO
尺寸
100 Tests
品类编号
562574
PE Mouse IgG1, κ Isotype Control RUO
尺寸
0.1 mg
品类编号
554680
Alexa Fluor®488 Mouse Anti-Human CD4 RUO
尺寸
100 Tests
品类编号
557695
Transcription Factor Buffer Set RUO
尺寸
25 Tests
品类编号
562725
565730 Rev. 1
抗体详情
6/hcKrox
The 6/hcKrox monoclonal antibody specifically recognizes human Th Inducing POZ-Kruppel Factor (ThPOK) which is also known as, cKrox or hcKrox. ThPOK is encoded by ZBTB7B (Zinc finger and BTB domain-containing protein 7B) and is a member of the POK/ZBT family of transcription regulators that play roles in lymphocyte development and the regulation of type I collagen genes. ThPOK expression is upregulated during the differentiation of CD4+ T helper cells but not CD8+ T cells. It indirectly increases the expression of CD4 in developing T cells by antagonizing Runx-3-mediated CD4 repression. ThPOK is also expressed in NKT and γδ T cells.
565730 Rev. 1
格式详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
565730 Rev.1
报价单和参考
View product citations for antibody "565730" on CiteAb
研发参考 (3)
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Heegaard AM, Gehron Robey P, Vogel W. Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox. J Bone Miner Res. 1997; 12(12):2050-2060. (Biology). 查看参考
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Peterkofsky B, Gosiewska A, Singh K, Pearlman S, Mahmoodian F. Species differences in cis-elements of the proalpha1(I) procollagen promoter and their binding proteins. J Cell Biochem. 1999; 73(3):408-422. (Biology). 查看参考
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Widom RL, Culic I, Lee JY, Korn JH. Cloning and characterization of hcKrox, a transcriptional regulator of extracellular matrix gene expression. Gene. 1997; 198(1-2):407-420. (Biology). 查看参考
565730 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.