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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Multicolor flow cytometric analysis of THEMIS expressed in human PBMC. Human peripheral blood mononuclear cells (PBMC) were isolated from freshly drawn EDTA-treated blood. The cells were fixed and permeabilized using reagents from the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). The cells were then stained with PE Mouse Anti-Human THEMIS (Cat. No. 562588) and PerCP-Cy™5.5 Mouse Anti-Human CD3 (Cat. No. 560835) antibodies. The two-color dot plot was derived from gated events with the forward and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.
BD Pharmingen™ PE Mouse anti-Human THEMIS
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准备和存储
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The Q13-1103 monoclonal antibody specifically binds to human THEMIS (Thymocyte-expressed molecule involved in selection). The human THEMIS gene encodes a 73-kDa signaling protein that is also known as thymocyte selection pathway associated (TSEPA), signaling phosphoprotein specific for T cells (SPOT) and GRB2-associated protein (GASP). The homologous mouse protein is encoded by the Themis gene. THEMIS mRNA is detected in the thymus, lymph nodes, spleen but not in the bone marrow or nonlymphoid tissues. It is expressed by all thymocytes including double- (high levels) and single-positive αβ T cells, γδ T cells and peripheral T cells (low levels) but not by B cells and NK cells. THEMIS plays a central role in T cell development including positive and negative selection processes in the thymus. It is involved in TCR signaling and gets phosphorylated quickly after TCR stimulation. In the mouse system, Themis-deficient double-positive thymocytes reportedly expressed lower levels of genes involved in later stages of T cell development including those related to cellular metabolism, cell cycle and survival functions. Themis-deficient thymocytes showed impaired selection that lead to the production of fewer mature thymocytes. The Q13-1103 antibody crossreacted with mouse Themis in Western blot testing of cellular lysates but did not crossreact when tested by immunofluorescent staining and flow cytometric analysis of cells.
研发参考 (4)
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Fu G, Vallée S, Rybakin V, et al. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat Immunol. 2009; 10(8):848-856. (Biology). 查看参考
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Johnson AL, Aravind L, Shulzhenko N, et al. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. Nat Immunol. 2009; 10(8):831-838. (Biology). 查看参考
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Lesourne R, Uehara S, Lee J, et al. Themis, a T cell-specific protein important for late thymocyte development. Nat Immunol. 2009; 10(8):840-847. (Biology). 查看参考
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Patrick MS, Oda H, Hayakawa K, et al. Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes. Proc Natl Acad Sci U S A. 2009; 106(38):16345-16350. (Biology). 查看参考
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