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Cell Preparation and Separation Reagents
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- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
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Two-parameter flow cytometric analysis of ILT1 (CD85h) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human ILT1 (CD85h) antibody (Cat. No. 566577; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of ILT1 (CD85h) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human ILT1 (CD85h)
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The monoclonal antibody 24 specifically recognizes Immunoglobulin-like transcript 1 (ILT1) which is also known as Leukocyte immunoglobulin-like receptor 7 (LIR-7). ILT1 (CD85h) is encoded by LILRA2 (Leukocyte immunoglobulin like receptor A2) and belongs to the Leukocyte immunoglobulin like receptors (LILR) gene family. This immunoreceptor is a ~69 kDa type I transmembrane glycoprotein that contains 4 Ig-like C2-type immunoglobulin-like extracellular domains and a short cytoplasmic domain. It is expressed on monocytes, macrophages, myeloid dendritic cells, and at lower levels on some granulocytes and a small subset of natural killer (NK) cells. ILT1 (CD85h) serves as an activating immunoreceptor through its short cytoplasmic domain that associates with the signal-transducing Fc receptor γ chain. This receptor complex mediates suppression of innate immune responses and inhibition of dendritic cell differentiation and antigen presentation. ILT1 (CD85h) may act as a receptor for immunoglobulins cleaved by microbial proteases.
研发参考 (5)
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Hirayasu K, Saito F, Suenaga T, et al. Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2. Nat Microbiol. 2016; 1(6):16054. (Biology). 查看参考
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Lee DJ, Sieling PA, Ochoa MT, et al. LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007; 179(12):8128-8136. (Immunogen: Functional assay, Immunofluorescence). 查看参考
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Lichterfeld M, Yu XG. The emerging role of leukocyte immunoglobulin-like receptors (LILRs) in HIV-1 infection. J Leukoc Biol. 2012; 91(1):27-33. (Biology). 查看参考
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Nakajima H, Samaridis J, Angman L, Colonna M. Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor gamma-chain.. J Immunol. 1999; 162(1):5-8. (Biology). 查看参考
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Tedla N, Bandeira-Melo C, Tassinari P, et al. Activation of human eosinophils through leukocyte immunoglobulin-like receptor 7. Proc Natl Acad Sci U S A. 2003; 100(3):1174-1179. (Biology). 查看参考
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