BD Pharmingen™ PE Mouse Anti-Human FCRL5 (CD307e)
克隆 509F6 (RUO)
Flow cytometric analysis of FCRL5 (CD307e) expression on transfectants and tonsillar B cells. Left Plot: Non-transfected (Dotted line histogram) and Human FCRL5-transfected (Solid line histogram) P815 cells were surface stained with PE Mouse Anti-Human FCRL5 (CD307e) (Cat. No. 566734) at 0.5 µg/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 (CD307e) on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 (Cat. No. 561742 or 555415) and either PE Mouse Anti-Human FCRL5 (CD307e) (Cat. No. 566734; Middle Plot) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 (CD307e) [or Ig isotype control staining] versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur™ Flow Cytometer System and FlowJo™ software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific.
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Flow cytometric analysis of FCRL5 (CD307e) expression on transfectants and tonsillar B cells. Left Plot: Non-transfected (Dotted line histogram) and Human FCRL5-transfected (Solid line histogram) P815 cells were surface stained with PE Mouse Anti-Human FCRL5 (CD307e) (Cat. No. 566734) at 0.5 µg/test. Flow cytometric fluorescence histograms showing the expression of FCRL5 (CD307e) on non-transfected or Human FCRL5-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Middle and Right Plots: Tonsil cells were treated with an Fc receptor blocker and stained with APC Mouse Anti-Human CD19 (Cat. No. 561742 or 555415) and either PE Mouse Anti-Human FCRL5 (CD307e) (Cat. No. 566734; Middle Plot) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of FCRL5 (CD307e) [or Ig isotype control staining] versus Human CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCalibur™ Flow Cytometer System and FlowJo™ software. Data courtesy of Dr. Marco Colonna, Washington University School of Medicine. Data shown on this Technical Data Sheet are not lot specific.
商品详情
BD Pharmingen™
BXMAS1; FCRH5; Fc receptor-like protein 5; IRTA2; PRO820; fc receptor homolog 5; fcR-like protein 5
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human FCRL5 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869836
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 mL
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
PE Mouse IgG2a, κ Isotype Control RUO
尺寸
0.1 mg
品类编号
554648
APC Mouse Anti-Human CD19 RUO
尺寸
25 Tests
品类编号
561742
APC Mouse Anti-Human CD19 RUO
尺寸
100 Tests
品类编号
555415
566734 Rev. 1
抗体详情
509F6
The 509F6 monoclonal antibody specifically binds to Fc receptor-like 5 (FCLR5), a type I transmembrane glycoprotein that is also known as CD307e, FCRH5 (Fc receptor homolog 5) and IRTA2 (immunoglobulin superfamily receptor translocation associated 2). FCRL5 (CD307e) is a member of the Immunoglobulin (Ig) gene superfamily with 9 extracellular Ig domains, an intracellular immunoreceptor tyrosine-based activating motif (ITAM)-like sequence and two intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM)-like sequences. Like other Fc receptor-like proteins, FCRL5 (CD307e) is homologous to FcγR1 (CD64) and binds to immunoglobulins, specifically the IgG isotypes, and it may play a role in the regulatory interactions of immune complexes with B lymphocytes. FCRL5 (CD307e) is differentially expressed in the B cell lineage, with little expression on peripheral blood cells and upregulated expression on plasma cells and some leukemias and lymphomas. mAb 509F6 binds to an epitope within the first 3 Ig domains of FCRL5 and blocks FCRL5's interaction with immune complexes.
566734 Rev. 1
格式详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566734 Rev.1
报价单和参考
View product citations for antibody "566734" on CiteAb
研发参考 (5)
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Damdinsuren B, Dement-Brown J, Li H, Tolnay M. B cell receptor induced Fc receptor-like 5 expression is mediated by multiple signaling pathways converging on NF-κB and NFAT. Mol Immunol. 2016; 73:112-21. (Clone-specific: Flow cytometry). 查看参考
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Li FJ, Won WJ, Becker EJ, et al. Emerging roles for the FCRL family members in lymphocyte biology and disease.. Curr Top Microbiol Immunol. 2014; 382:29-50. (Biology). 查看参考
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Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016; 196(10):4064-74. (Clone-specific: Flow cytometry). 查看参考
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Polson AG, Zheng B, Elkins K, et al. Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia. Int Immunol. 2006; 18(9):1363-1373. (Biology). 查看参考
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Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012; 188(10):4741-4745. (Immunogen: Blocking, Flow cytometry). 查看参考
566734 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.