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Flow cytometry analysis of CD34 expression on Rhesus macaque (Macaca mulatta) stem cells (CD14-negative) from peripheral blood mononuclear cells. Rhesus PBMCs were stained with V450 Mouse Anti-Human CD14 (Cat. No. 561390) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; data not shown) or PE Mouse Anti-Human CD34 (Cat. No. 550619/550761). Fluorescent dot plot depicting CD34 expression was derived from gated events with the forward and side light-scatter characteristics of viable, CD14-negative cells.
BD Pharmingen™ PE Mouse Anti-Human CD34
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- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
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The 563 monoclonal antibody specifically binds to CD34. CD34 is a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 563 belongs to the class III epitope; it is resistant to neuraminidase, chymopapain and glycoprotease. This antibody is able to block the binding of other CD34 mAbs like 581.
Clone 563 reacts with the human form of the 105-120 kDa heavily O-glycosylated and N-glycosylated transmembrane glycoprotein, CD34, expressed on stem/progenitor hematopoietic cells, endothelial cells and some tissue. Clone 563 also cross reacts with a subset of peripheral blood mononuclear cells of both rhesus and cynomolgus macaque monkeys, but not baboon. The distribution of stem-like cells is similar to that observed with peripheral blood mononuclear cells from normal human donors.
研发参考 (6)
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Egeland T, Tjonnfjord G, Steen R, Gaudernack G, Thorsby E. Positive selection of bone marrow-derived CD34 positive cells for possible stem cell transplantation. Transplant Proc. 1993; 25(1):1261-1263. (Biology). 查看参考
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Nishio H, Tada J, Hashiyama N, Hirn J, Ingles-Esteven J, Suda T. CD34. 1999. Available: http://mpr.nci.nih.gov/prow/guide/968267813_g.htm 2006, February 8.
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Owens MA, Loken MR. Peripheral blood stem cell quantitation. In: Owens MA, Loken MR. Flow Cytometry Principles for Clinical Laboratory Practice. New York: John Wiley & Sons; 1995:128.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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