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Flow cytometric analysis of human CD334 expression on human MDA-MB-453 cells. Cells from the human MDA-MB-453 (Breast adenocarcinoma, ATCC HTB-131) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or Mouse Anti-Human CD334 antibody (Cat. No. 566662; solid line histogram) at 0.25 µg/test. The flow cytometric histogram showing CD334 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human CD334
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 4FR6D3 monoclonal antibody specifically recognizes CD334 which is also known as Fibroblast growth factor receptor 4 (FGFR4), Tyrosine kinase related to fibroblast growth factor receptor (TKF), or JTK2. CD334 is a ~110 kDa type I transmembrane glycoprotein that belongs to the fibroblast growth factor receptor (FGFR) in the Ig superfamily (IgSF). The ligand-binding extracellular domain has three immunoglobulin-like C2-type domains followed by a transmembrane region and a cytoplasmic tail which contains a tyrosine kinase domain. CD334 serves as a high affinity receptor that dimerizes upon binding to fibroblast growth factors including FGF-1, -2, -4, -6, -8, -9, -16, -17, -18, and -19. This cell surface receptor tyrosine kinase is widely expressed on epithelial cells, fibroblasts and skeletal muscle cells and plays a role in the proliferation, differentiation, migration, and survival of these different cell types. Abnormal expression and activity of CD334 has been associated with a number of different cancers.
研发参考 (3)
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Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE. A novel marker of human endometrial mesenchymal stem-like cells.. Cell Transplant. 2012; 21(10):2201-14. (Clone-specific: Flow cytometry, Immunohistochemistry). 查看参考
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Tiong KH, Mah LY, Leong CO. Functional roles of fibroblast growth factor receptors (FGFRs) signaling in human cancers. Apoptosis. 2013; 18(12):1447-1468. (Biology). 查看参考
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Zola H, Swart B, Nicholson I, Voss E. CD334. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:534-535.
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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