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Reagents
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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
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- BD® OMICS-Guard Sample Preservation Buffer
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Flow cytometric analysis of CD234 expression on human peripheral blood erythrocytes. Human peripheral blood erythrocytes were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; dashed line histogram) or PE Mouse Anti-Human CD234 antibody (Cat. No. 566424; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side-light scattering characteristics of erythrocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human CD234 (DARC)
监管状态图例
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准备和存储
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The NaM185-2C3 monoclonal antibody specifically recognizes CD234 which is also known as the Duffy antigen/receptor for chemokines (DARC), Fy glycoprotein (FY), or Glycoprotein D (GPD). CD234 is a seven-transmembrane domain acidic glycoprotein that is encoded by ACKR1 [atypical chemokine receptor 1 (Duffy blood group)] and belongs to the chemokine receptor superfamily. CD234 is responsible for the Duffy blood group system and is expressed on erythrocytes, endothelial cells, and some epithelial cells and Purkinje cells. CD234 binds to multiple chemokines including CXCL1 (also known as, MGSA or GROa), CXCL8 (IL-8), CCL2 (MCP-1), and CCL5 (RANTES). Because CD234 is not a signaling receptor, it may act as a decoy receptor or scavenger and serve to control chemokine levels. CD234 may also serve to regulate the availability of chemokines such as when it is expressed by endothelial cells at inflammatory sites or by postcapillary endothelial cells. CD234 can mediate chemokine transcytosis from the basolateral to the luminal side of the endothelium, where the bound chemokines are "presented" to regulate leucocyte trafficking. CD234 functions as a receptor for malarial parasites including Plasmodium vivax and Plasmodium knowlesi. Some individuals with mutations that prevent CD234 expression in erythrocytes are resistant to infection by these pathogens. The NaM185-2C3 antibody reportedly detects a protease-sensitive determinant and inhibits IL-8 binding to the Duffy antigen.
研发参考 (9)
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Blanchard D, Petit-Le Roux Y, Tournamille C, Folléa G. Characterization of monoclonal antibodies directed against the Fy/DARC erythrocyte glycoprotein. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:574-576. 查看参考
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Geoff Daniels. Duffy Blood Group System. In: Daniels G. Human blood groups : Geoff Daniels ; foreword by Ruth Sanger., 3rd ed.. Chichester, West Sussex: John Wiley & Sons; 2013:306.
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Horuk R, Chitnis CE, Darbonne WC, et al. A receptor for the malarial parasite Plasmodium vivax: the erythrocyte chemokine receptor.. Science. 1993; 261(5125):1182-4. (Biology). 查看参考
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Nichols ME, Rubinstein P, Barnwell J, Rodriguez de Cordoba S, Rosenfield RE. A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax.. J Exp Med. 1987; 166(3):776-85. (Biology). 查看参考
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Riwom S, Janvier D, Navenot JM, Benbunan M, Muller JY, Blanchard D. Production of a new murine monoclonal antibody with Fy6 specificity and characterization of the immunopurified N-glycosylated Duffy-active molecule.. Vox Sang. 1994; 66(1):61-7. (Biology). 查看参考
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Van der Schoot CE, Baardman R, Lighthart P, de Jong I, Eg Von Dem Borne A, de Haas M. CD234 - DARC. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:573-574.
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Walker RH, ed. Technical Manual of the American Association of Blood Banks. Arlington VA: 1990.
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Wasniowska K, Petit-LeRoux Y, Tournamille C, et al. Structural characterization of the epitope recognized by the new anti-Fy6 monoclonal antibody NaM 185-2C3.. Transfus Med. 2002; 12(3):205-11. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Hemagglutination assay, Inhibition, Western blot). 查看参考
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Zola H, Swart B, Nicholson I, Voss E. CD234. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:387.
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