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PE_on_HEL.png

Flow cytometric analysis of human CD110 expression on platelets and HEL92.1.7 cells. Platelets (Left Panel) and HEL92.1.7 cells (Right Panel) were stained with either PE Mouse IgG2b Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse anti-Human CD110 (Cat. No. 562159; solid line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of platelets or viable HEL92.1.7 cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

562159Image1.png

Flow cytometric analysis of human CD110 expression on platelets and HEL92.1.7 cells. Platelets (Left Panel) and HEL92.1.7 cells (Right Panel) were stained with either PE Mouse IgG2b Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse anti-Human CD110 (Cat. No. 562159; solid line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of platelets or viable HEL92.1.7 cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

PE_on_HEL.png 562159Image1.png Red-Cap-Blue-Label.jpg

Flow cytometric analysis of human CD110 expression on platelets and HEL92.1.7 cells. Platelets (Left Panel) and HEL92.1.7 cells (Right Panel) were stained with either PE Mouse IgG2b Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse anti-Human CD110 (Cat. No. 562159; solid line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of platelets or viable HEL92.1.7 cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of human CD110 expression on platelets and HEL92.1.7 cells. Platelets (Left Panel) and HEL92.1.7 cells (Right Panel) were stained with either PE Mouse IgG2b Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse anti-Human CD110 (Cat. No. 562159; solid line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of platelets or viable HEL92.1.7 cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

商品详情
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BD Pharmingen™
C-MPL; MPL; MPLV; TPOR; Thrombopoietin receptor
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10893807
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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562159 Rev. 3
抗体详情
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1.6.1

The 1.6.1 monoclonal antibody specifically binds to the human Thrombopoietin Receptor (TPO-R) that is also known as the Myeloproliferative leukemia protein (c-Mpl) or CD110. CD110 is a type I transmembrane glycoprotein and a member of the hematopoietin receptor family. It is expressed on hematopoietic stem cells, a subfraction of hematopoietic precursor cells, cells of the megakaryocytic lineage and platelets. CD110 serves as a receptor for thrombopoietin. Upon binding of thrombopoietin to CD110, megakaryocyte proliferation and differentiation is induced, platelets are produced and stem cells are protected from apoptosis.

562159 Rev. 3
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
562159 Rev.3
报价单和参考
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View product citations for antibody "562159" on CiteAb

研发参考 (5)

  1. Abbott C, Huang G, Ellison AR, et al. Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications.. Hybridoma (Larchmt). 2010; 29(2):103-13. (Clone-specific: Flow cytometry). 查看参考
  2. Broudy VC, Lin NL, Fox N, Taga T, Saito M, Kaushansky K. Thrombopoietin stimulates colony-forming unit-megakaryocyte proliferation and megakaryocyte maturation independently of cytokines that signal through the gp130 receptor subunit. Blood. 1996; 88(6):2026-2032. (Biology). 查看参考
  3. Deng B, Banu N, Malloy B, et al. An agonist murine monoclonal antibody to the human c-Mpl receptor stimulates megakaryocytopoiesis. Blood. 1998; 92(6):1981-1988. (Biology). 查看参考
  4. Fox NE, Lim J, Chen R, Geddis AE. F104S c-Mpl responds to a transmembrane domain-binding thrombopoietin receptor agonist: proof of concept that selected receptor mutations in congenital amegakaryocytic thrombocytopenia can be stimulated with alternative thrombopoietic agents. Exp Hematol. 2010; 38(5):384-391. (Clone-specific: Flow cytometry). 查看参考
  5. Gotoh A, Ritchie A, Takahira H, Broxmeyer HE. Thrombopoietin and erythropoietin activate inside-out signaling of integrin and enhance adhesion to immobilized fibronectin in human growth-factor-dependent hematopoietic cells. Ann Hematol. 1997; 75(5-6):207-213. (Biology). 查看参考
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562159 Rev. 3

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.