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Reagents
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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Two-color analysis of the expression of Vα 3.2b,c TCR on peripheral lymphocytes. C57BL/6 lymph node leukocytes were simultaneuosly stained with FITC-conjugated RR3-16, PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ FITC Rat Anti-Mouse Vα 3.2[b,c] TCR
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The RR3-16 monoclonal antibody specifically reacts with the Vα 3.2 T-Cell Receptor (TCR) of mice having the b (e.g., C57BL) and c (e.g., SWR, SJL, NZB, NOD) haplotypes of the Tcra gene complex, but not with TCR encoded by other members of Tcra-V3 gene subfamily. RR3-16 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or d (e.g., DBA/1, DBA/2, NZW) Tcra haplotypes. In addition, it has been shown that frequencies of Vα 3.2+ CD8+ T cells from homozygous H-2k/H-2k mice are moderately higher than those from heterozygous H-2k/H-2d mice, suggesting positive selection by H-2k.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (3)
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Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). 查看参考
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Utsunomiya Y, Bill J, Palmer E, Gollob K, Takagaki Y, Kanagawa O. Analysis of a monoclonal rat antibody directed to the alpha-chain variable region (V alpha 3) of the mouse T cell antigen receptor. J Immunol. 1989; 143(8):2602-2608. (Immunogen). 查看参考
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Utsunomiya Y, Bill J, Palmer E, Kanagawa O. Identification of a mouse T-cell antigen receptor alpha-chain polymorphism by a V alpha 3.2 chain-specific monoclonal antibody. Immunogenetics. 1991; 33(3):198-201. (Clone-specific). 查看参考
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