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      FITC Mouse Anti-Pig CD8a
      FITC Mouse Anti-Pig CD8a
      CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained with either FITC-conjugated 76-2-11 mAb (shaded histogram) or FITC-conjugated mouse Igg2a, kappa isotype control mAb G155-178 (Cat. No. 553456, open histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      FITC Mouse Anti-Pig CD8a
      CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained with either FITC-conjugated 76-2-11 mAb (shaded histogram) or FITC-conjugated mouse Igg2a, kappa isotype control mAb G155-178 (Cat. No. 553456, open histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      商品详情
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      BD Pharmingen™
      Pig (QC Testing)
      Mouse BALB/c IgG2a, κ
      dd miniature swine thymocytes
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_2291363
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      551303 Rev. 3
      抗体详情
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      76-2-11

      The 76-2-11 (also known as clone PT8) monoclonal antibody specifically binds to an epitope on the CD8α chain, a 35-kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and β chain. Cells which express the CD8αα homodimer display dimmer staining with mAb 76-2-11 than CDαβ-expressing cells. The 76-2-11 mAb does not cross-react with human or bovine cells. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 76-2-11 has been reported to partially inhibit in vitro cytotoxic activity of PBL to allogeneic leukocytes, but not NK-cell-mediated lysis, and to deplete CD8+ T cells in vivo. This clone was clustered as anti-CD8a at the First International Swine CD workshop.

      551303 Rev. 3
      格式详情
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      551303 Rev.3
      报价单和参考
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      View product citations for antibody "551303" on CiteAb

      研发参考 (13)

      1. Dato ME, Kim YB. Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells. J Immunol. 1990; 144(11):4452-4462. (Biology). 查看参考
      2. Pescovitz MD, Lowman MA, Sachs DH. Expression of T-cell associated antigens by porcine natural killer cells. Immunology. 1988; 65(2):267-271. (Biology). 查看参考
      3. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985; 134(1):37-44. (Clone-specific). 查看参考
      4. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Immunogen). 查看参考
      5. Saalmuller A, Aasted B, Canals A, et al. Analyses of mAb reactive with porcine CD8. Vet Immunol Immunopathol. 1994; 43(1-3):249-254. (Clone-specific). 查看参考
      6. Saalmuller A, Hirt W, Maurer S, Weiland E. Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen. Immunology. 1994; 81(4):578-583. (Biology). 查看参考
      7. Saalmuller A, Pauly T, Hohlich BJ, Pfaff E. Characterization of porcine T lymphocytes and their immune response against viral antigens. J Biotechnol. 1999; 73(2-3):223-233. (Biology). 查看参考
      8. Saalmuller A, Werner T, Fachinger V. T-helper cells from naive to committed. Vet Immunol Immunopathol. 2002; 87(3-4):137-145. (Biology). 查看参考
      9. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol. 1994; 16(2):105-114. (Biology). 查看参考
      10. Suzuki T, Sundt TM 3rd, Mixon A, Sachs DH. In vivo treatment with antiporcine T cell antibodies. Transplantation. 1990; 50(1):76-81. (Biology). 查看参考
      11. Waters WR, Hontecillas R, Sacco RE, et al. Antigen-specific proliferation of porcine CD8alphaalpha cells to an extracellular bacterial pathogen. Immunology. 2000; 101(3):333-341. (Clone-specific). 查看参考
      12. Zuckermann FA, Pescovitz MD, Aasted B, et al. Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop. Vet Immunol Immunopathol. 1998; 60(3-4):291-303. (Clone-specific). 查看参考
      13. Zuckermann FA. Extrathymic CD4/CD8 double positive T cells. Vet Immunol Immunopathol. 1999; 72(1-2):55-66. (Biology). 查看参考
      查看所有文件 (13) 查看更少内容
      551303 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.