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      FITC Mouse Anti-Mouse Vβ 13 TCR
      FITC Mouse Anti-Mouse Vβ 13 TCR
      Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
      FITC Mouse Anti-Mouse Vβ 13 TCR
      Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
      商品详情
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      BD Pharmingen™
      TCR V beta 13; TCR Vβ 13; TCR Vβ13; Vβ 13 T-cell Receptor
      Mouse (QC Testing)
      Mouse C57BR IgG1, κ
      Not reported
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394706
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      推荐的实验流程

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553204 Rev. 10
      抗体详情
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      MR12-3

      The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.

      553204 Rev. 10
      格式详情
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      553204 Rev.10
      报价单和参考
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      研发参考 (3)

      1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). 查看参考
      2. Chen D, Lee F, Cebra JJ, Rubin DH. Predominant T-cell receptor Vbeta usage of intraepithelial lymphocytes during the immune response to enteric reovirus infection.. J Virol. 1997; 71(5):3431-6. (Biology). 查看参考
      3. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). 查看参考
      553204 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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