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FITC Mouse Anti-Mouse H-2D[b]
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BD Pharmingen™
Histocompatibility-2Db; H-2Db
Mouse (QC Testing)
Mouse BALB/c IgG2b, κ
C57BL/10 mouse skin graft and splenocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
553573 Rev. 2
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The KH95 antibody specifically recognizes the H-2D[b] MHC class I alloantigen. Reactivity with other haplotypes (e.g., a, d, f, k, n, p, q, r, s, u, v) has not been observed.

553573 Rev. 2
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
553573 Rev.2
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View product citations for antibody "553573" on CiteAb

研发参考 (1)

  1. Hasenkrug KJ, Cory JM, Stimpfling JH. Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region. Immunogenetics. 1987; 25(2):136-139. (Immunogen). 查看参考
553573 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.