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      FITC Mouse Anti-Human CD62P
      FITC Mouse Anti-Human CD62P
      Flow cytometric analysis of CD62P expression on activated Cynomologus macaque (Macaca fascicularis) platelets. Platelets were stained with either FITC Mouse Anti-Human CD62P (Cat. No. 550866; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 556649; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable platelets.
      FITC Mouse Anti-Human CD62P
      Flow cytometric analysis of CD62P expression on activated Cynomologus macaque (Macaca fascicularis) platelets. Platelets were stained with either FITC Mouse Anti-Human CD62P (Cat. No. 550866; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 556649; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable platelets.
      商品详情
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      BD Pharmingen™
      SELP; PSEL; P-selectin; PADGEM; LECAM3; GMP-140; GRMP; GMP140
      Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Activated Human Platelets
      Flow cytometry (Routinely Tested)
      20 µl
      V S058
      6403
      AB_393934
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550866 Rev. 4
      抗体详情
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      AC1.2

      The AC1.2 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin may mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.

      550866 Rev. 4
      格式详情
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      550866 Rev.4
      报价单和参考
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      View product citations for antibody "550866" on CiteAb

      研发参考 (13)

      1. Albelda SM. Role of integrins and other cell adhesion molecules in tumor progression and metastasis. Lab Invest. 1993; 68(1):4-17. (Biology). 查看参考
      2. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology). 查看参考
      3. Dore M, Korthuis RJ, Granger DN, Entman ML, Smith CW. P-selectin mediates spontaneous leukocyte rolling in vivo. Blood. 1993; 82(4):1308-1316. (Biology). 查看参考
      4. Groves RW, Allen MH, Ross EL, Ahsan G, Barker JN, MacDonald DM. Expression of selectin ligands by cutaneous squamous cell carcinoma. Am J Pathol. 1993; 143(4):1220-1225. (Biology). 查看参考
      5. Johnson-Tidey RR, McGregor JL, Taylor PR, Poston RN. Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 1994; 144(5):952-961. (Biology). 查看参考
      6. Kansas GS. Selectins and their ligands: current concepts and controversies. Blood. 1997; 88(9):3259-3287. (Biology). 查看参考
      7. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
      8. Larsen E, Celi A, Gilbert GE, et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.. Cell. 1989; 59(2):305-12. (Immunogen). 查看参考
      9. Lorant DE, Topham MK, Whatley RE, et al. Inflammatory roles of P-selectin. J Clin Invest. 1993; 92(2):559-570. (Biology). 查看参考
      10. Nagata K, Tsuji T, Todoroki N, et al. Activated platelets induce superoxide anion release by monocytes and neutrophils through P-selectin (CD62). J Immunol. 1993; 151(6):3267-3273. (Biology). 查看参考
      11. Pigott R, Power C. The Adhesion Molecule Facts Book. 1993:173.
      12. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      13. Stone JP, Wagner DD. P-selectin mediates adhesion of platelets to neuroblastoma and small cell lung cancer. J Clin Invest. 1993; 92(2):804-813. (Biology). 查看参考
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      550866 Rev. 4

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.