FITC Mouse Anti-Human CD147

BD Pharmingen™ FITC Mouse Anti-Human CD147

Name: FITC Mouse Anti-Human CD147
Brand: FITC Mouse Anti-Human CD147
SKU: 555962

克隆 HIM6 (RUO)

Profile of peripheral blood lymphocytes analyzed by flow cytometry.

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实验方案

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: BSG; Basigin; BASI; Neurothelin; 5F7; EMMPRIN; M6; OK; TCSF

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VI NL109

RRID: AB_396256

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

配套商品

FITC Mouse IgG1, κ Isotype Control RUO

尺寸 100 Tests 品类编号 555748

555962 Rev. 6

抗体详情

HIM6

The HIM6 monoclonal antibody specifically binds to CD147 which is encoded by BSG. CD147 is a type I transmembrane glycoprotein (30-50 kDa) of the immunoglobulin super-gene family. Neurothelin, a blood-brain barrier-specific molecule, was clustered as CD147 in the Sixth Human Leukocyte Differentiation Antigen (HLDA) workshop. It bears homology with mouse gp42 or basigin, human "M6" or "EMMPRIN", rat OX-47 or CD-9, and avian HT7 or 5A11. CD147 is also known as Tumor cell-derived collagenase stimulatory factor (TCSF). CD147 is a molecule that is broadly expressed on cells of hematopoietic and non-hematopoietic origin. Its expression on specific cell types may be regulated by cytokines. CD147 plays a role in embryonal blood-brain barrier development and a role in integrin-mediated adhesion in brain endothelia.

555962 Rev. 6

格式详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: FITC

激发源: Blue 488 nm

最多激发源: 494 nm

最多发射源: 518 nm

555962 Rev.6

报价单和参考

View product citations for antibody "555962" on CiteAb

研发参考 (5)

Fadool JM, Linser PJ. Evidence for the formation of multimeric forms of the 5A11/HT7 antigen. Biochem Biophys Res Commun. 1996; 229(1):280-286. (Biology). 查看参考
Ikeda E, Flamme I, Risau W. Developing brain cells produce factors capable of inducing the HT7 antigen, a blood-brain barrier-specific molecule, in chick endothelial cells. Neurosci Lett. 1996; 209(3):149-152. (Biology). 查看参考
Schuster VL, Lu R, Kanai N, et al. Cloning of the rabbit homologue of mouse 'basigin' and rat 'OX-47': kidney cell type-specific expression, and regulation in collecting duct cells. Biochim Biophys Acta. 1996; 1311(1):13-19. (Biology). 查看参考
Stockinger H, Ebel T, Hansmann C, et al.. EC16 CD147 (neurothelin/basigin) Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:760-763.
Sudou A, Ozawa M, Muramatsu T. Lewis X structure increases cell substratum adhesion in L cells. J Biochem (Tokyo). 1995; 117(2):271-275. (Biology). 查看参考

查看所有文件 (5)

555962 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.