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BV421 Rat Anti-Mouse CD68
商品详情
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BD Horizon™
Cd68; Macrosialin; Lamp4; gp110; Scard1
Mouse (QC Testing)
Rat IgG2a, κ
Purified Con A Acceptor Glycoproteins from the Mouse P815 Cell Line
Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Tested During Development)
0.2 mg/ml
AB_2744447
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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推荐的实验流程

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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566388 Rev. 1
抗体详情
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FA/11

The FA/11 monoclonal antibody specifically binds to CD68 which is also known as Macrosialin, Lamp4 or Scard1. CD68 is a heavily glycosylated ~ 85-115 kDa type I transmembrane protein that belongs to the lysosomal-associated membrane protein (LAMP) family. Although it is expressed at the cell surface, it is predominantly expressed as an intracellular protein in late endosomes. CD68 is expressed by macrophages, Kupffer cells, histiocytes, microglia, and is considered a useful pan-macrophage marker. It is also expressed by dendritic cells, Langerhans cells and osteoclasts. CD68 expression is upregulated and its glycosylation patterns can be changed by proinflammatory agents. Although CD68 binds to oxidized low-density lipoproteins, its exact biological function is not well defined.

566388 Rev. 1
格式详情
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
407 nm
423 nm
566388 Rev.1
报价单和参考
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View product citations for antibody "566388" on CiteAb

研发参考 (6)

  1. Ashley JW, Shi Z, Zhao H, Li X, Kesterson RA, Feng X. Genetic ablation of CD68 results in mice with increased bone and dysfunctional osteoclasts.. PLoS ONE. 2011; 6(10):e25838. (Clone-specific: Flow cytometry, Immunofluorescence, Western blot). 查看参考
  2. Kurushima H, Ramprasad M, Kondratenko N, Foster DM, Quehenberger O, Steinberg D. Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages.. J Leukoc Biol. 2000; 67(1):104-8. (Biology). 查看参考
  3. Rabinowitz SS, Gordon S. Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli.. J Exp Med. 1991; 174(4):827-36. (Clone-specific: Depletion, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western blot). 查看参考
  4. Ramprasad MP, Terpstra V, Kondratenko N, Quehenberger O, Steinberg D. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein.. Proc Natl Acad Sci USA. 1996; 93(25):14833-8. (Clone-specific: Flow cytometry). 查看参考
  5. Smith MJ, Koch GL. Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.. J Cell Sci. 1987; 87 ( Pt 1):113-9. (Immunogen: Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Radioimmunoassay). 查看参考
  6. Song L, Lee C, Schindler C. Deletion of the murine scavenger receptor CD68.. J Lipid Res. 2011; 52(8):1542-50. (Clone-specific: Flow cytometry). 查看参考
查看所有文件 (6) 查看更少内容
566388 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.