BV421 Rat Anti-Human Cutaneous Lymphocyte Antigen

BD Horizon™ BV421 Rat Anti-Human Cutaneous Lymphocyte Antigen

Name: BV421 Rat Anti-Human Cutaneous Lymphocyte Antigen
Brand: BV421 Rat Anti-Human Cutaneous Lymphocyte Antigen
SKU: 563961

克隆 HECA-452 (RUO)

Multiparameter flow cytometric analysis of Cutaneous Lymphocyte Antigen expression on human peripheral blood lymphocytes. Whole blood was stained with either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708; Left Panel) or BD Horizon™ BV421 Rat Anti-Human Cutaneous Lymphocyte Antigen antibody (Cat. No. 563961; Right Panel). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Two-parameter flow cytometric dot plots show the correlated expression patterns of Cutaneous Lymphocyte Antigen (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals for gated events with the light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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商品详情

Brand: BD Horizon™

Alternative Name: CLA; PSGL-1; CD162; P-selectin glycoprotein ligand 1; SELPL; SELPLG

Reactivity: Human (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgM, κ

Immunogen: Lymphocyte-depleted Stromal Preparation of Tonsil

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: V S075

RRID: AB_2738513

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Brilliant Violet™ 421 is a trademark of Sirigen.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 mL 品类编号 554657

Lysing Solution 10X Concentrate CFDA_IVD

尺寸 100 mL 品类编号 349202

Lysing Buffer RUO

尺寸 100 mL 品类编号 555899

BV421 Rat IgM, κ Isotype Control RUO

尺寸 50 µg 品类编号 562708

563961 Rev. 1

抗体详情

HECA-452

The HECA-452 monoclonal antibody specifically reacts with Cutaneous Lymphocyte Associated Antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as a ligand for selectins including CD62E (E-selectin; ELAM-1). CLA is expressed on high endothelium and on lymphocytes including most T lymphocytes infiltrating cutaneous sites of inflammation. Amongst peripheral blood cells, it is expressed on monocytes and granulocytes and a subset of lymphocytes. The HECA-452 antibody is also reportedly crossreactive with the mouse CLA carbohydrate epitope that is transiently expressed by PSGL-1/CD162 on activated T cells. A number of studies suggest that CLA plays an important role in supporting leucocyte adhesive interactions and migration into extravascular tissues during inflammation.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

563961 Rev. 1

格式详情

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: BV421

激发源: Violet 405 nm

最多激发源: 407 nm

最多发射源: 423 nm

563961 Rev.1

报价单和参考

View product citations for antibody "563961" on CiteAb

研发参考 (7)

Autschbach F, Qiao L, Schürmann G, et al. Immunohistochemical reactivity of adhesion structure section mAb (Subpanels 2-4) in peripheral and mucosa-associated lymphoid tissue and in Crohn's disease. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1508-1510.
Berg EL, Yoshino T, Rott LS, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991; 174(6):1461-1466. (Clone-specific: Blocking, Flow cytometry). 查看参考
Borges E, Pendl G, Eytner R, Steegmaier M, Zollner O, Vestweber D. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997; 272(45):28786-28792. (Clone-specific: Blocking, Flow cytometry, Western blot). 查看参考
Duijvestijn AM, Horst E, Pals ST, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988; 130(1):147-155. (Immunogen: Immunohistochemistry). 查看参考
Picker LJ, Kishimoto TK, Smith CW, Warnock RA, Butcher EC. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991; 349(6312):796-799. (Clone-specific). 查看参考
Picker LJ, Warnock RA, Burns AR, Doerschuk CM, Berg EL, Butcher EC. The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell. 1991; 66(5):921-933. (Biology). 查看参考
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

查看所有文件 (7)

563961 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.