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Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human TRAIL-R1 (CD261) antibody (Cat. No. 744791; solid line histogram) on live HEL cells, with Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
BD OptiBuild™ BV421 Mouse Anti-Human TRAIL-R1 (CD261)
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
商品通知
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
配套商品
The S35-934 monoclonal antibody specifically binds to TNF-related apoptosis inducing ligand receptor 1 (TRAIL-R1/TRAIL Receptor 1). TRAIL-R1 is also known as CD261, Death Receptor 4 (DR4) and APO2. TRAIL-R1 is a type I transmembrane protein that is encoded by TNFRSF10A (tumor necrosis factor receptor superfamily, member 10a). It serves as a receptor for TNF-related apoptosis-inducing ligand (TRAIL) which is also known as CD253 and TNFSF10. TRAIL-R1 is expressed by a variety of tumor-derived cell lines. It is generally expressed at very low levels by most leucocytes. When bound by TRAIL, TRAIL-R1 can promote recruitment and subsequent activation of caspase-8, ultimately triggering the activation of effector caspases within cells that lead to apoptosis. Many transformed cell types are susceptible to TRAIL:TRAIL-R1-mediated cell death whereas normal cells are typically resistant.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
研发参考 (8)
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Dickens LS, Boyd RS, Jukes-Jones R, et al. A death effector domain chain DISC model reveals a crucial role for caspase-8 chain assembly in mediating apoptotic cell death. Mol Cell. 2012; 47(2):291-305. (Biology). 查看参考
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Falschlehner C, Schaefer U, Walczak H. Following TRAIL's path in the immune system. Immunology. 2009; 127(2):145-154. (Biology). 查看参考
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Florian S, Sonneck K, Czerny M, et al. Detection of novel leukocyte differentiation antigens on basophils and mast cells by HLDA8 antibodies. Allergy. 2006; 61(9):1054-1062. (Biology). 查看参考
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Grewal IS. Overview of TNF superfamily: a chest full of potential therapeutic targets. Adv Exp Med Biol. 2009; 647:1-7. (Biology). 查看参考
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Johnstone RW, Frew AJ, Smyth MJ. The TRAIL apoptotic pathway in cancer onset,progression and therapy. Nat Rev Cancer. 2008; 8(10):782-798. (Biology). 查看参考
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Pan G, O'Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL. Science. 1997; 276(5309):111-113. (Biology). 查看参考
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Spierings DC, de Vries EG, Vellenga E, et al. Tissue distribution of the death ligand 2 TRAIL and its receptors. J Histochem Cytochem. 2004; 52(6):821-831. (Biology). 查看参考
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Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 1995; 3(6):673-682. (Biology). 查看参考
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.