Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      BV421 Mouse Anti-Human ILT7 (CD85g)
      BV421 Mouse Anti-Human ILT7 (CD85g)
      Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood cells. Human peripheral blood mononuclear cells were stained with FITC Lineage Cocktail 1 (lin 1) (Cat. No. 340546), PerCP-Cy™5.5 Mouse Anti-Human CD123 antibody (Cat. No. 558714/560904) and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Panel) or BD Horizon™ BV421 Mouse Anti-Human ILT7 (CD85g) (Cat. No. 562578; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus ILT7 (or Ig Isotype control) for Lin-1 negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Mouse Anti-Human ILT7 (CD85g)
      Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood cells. Human peripheral blood mononuclear cells were stained with FITC Lineage Cocktail 1 (lin 1) (Cat. No. 340546), PerCP-Cy™5.5 Mouse Anti-Human CD123 antibody (Cat. No. 558714/560904) and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Panel) or BD Horizon™ BV421 Mouse Anti-Human ILT7 (CD85g) (Cat. No. 562578; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus ILT7 (or Ig Isotype control) for Lin-1 negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      商品详情
      Down Arrow Up Arrow


      BD Horizon™
      LILRA4; CD85g; ILT7; ILT-7; Immunoglobulin-like transcript 7
      Human (QC Testing)
      Mouse IgG1, κ
      ILT7–FcεRIγ-transfected 2B4 cells
      Flow cytometry (Routinely Tested)
      5 µl
      23547
      AB_2737662
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      推荐的实验流程

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD Optibuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Cy is a trademark of GE Healthcare.
      10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      562578 Rev. 3
      抗体详情
      Down Arrow Up Arrow
      17G10.2

      The 17G10.2 monoclonal antibody specifically binds to human Immunoglobulin Like Transcript 7 (ILT7), also known as CD85g. The ILT7 protein is encoded by the LILRA4 (Leukocyte Immunoglobulin Like Receptor subfamily A member 4) gene. ILT7 is a member of immunoglobulin-like transcripts (ILT) or leukocyte immunoglobulin-like receptor (LIR) gene family. ILT7 is selectively expressed on plasmacytoid dendrtic cells (pDC) but not on myeloid dendritic cells or other peripheral blood leukocytes. In response to IL-3, IL-3 receptor complex signaling downregulates ILT7 expression by pDC. ILT7 associates with the FcεRIγ signal adapter protein to form a receptor complex that can signal through activation of an immunoreceptor-based tyrosine activation motif (ITAM)-mediated signaling pathway. Signaling through the ILT7-FcεRIγ receptor complex negatively regulates the innate immune functions of pDC. ILT7 crosslinking inhibits TLR responses by pDC such as the stimulated production of type I interferon and other cytokines. Bone marrow stromal cell antigen 2 (BST2) has been identified as a biological ligand for ILT7.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

      562578 Rev. 3
      格式详情
      Down Arrow Up Arrow
      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      562578 Rev.3
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (3)

      1. Cao W, Bover L, Cho M, et al. Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction.. J Exp Med. 2009; 206(7):1603-14. (Clone-specific: Activation, Calcium Flux, Flow cytometry, Inhibition). 查看参考
      2. Cao W, Bover L. Signaling and ligand interaction of ILT7: receptor-mediated regulatory mechanisms for plasmacytoid dendritic cells. Immunol Rev. 2010; 234(1):163-176. (Clone-specific: Inhibition). 查看参考
      3. Cao W, Rosen DB, Ito T, et al. Plasmacytoid dendritic cell-specific receptor ILT7-Fc epsilonRI gamma inhibits Toll-like receptor-induced interferon production.. J Exp Med. 2006; 203(6):1399-405. (Immunogen: Activation, Calcium Flux, Flow cytometry, Functional assay, Inhibition). 查看参考
      562578 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback