-
Reagents
- Flow Cytometry Reagents
-
蛋白质印迹试剂
- 免疫分析 试剂
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
-
Functional Assays
-
显微成像试剂
-
Cell Preparation and Separation Reagents
-
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- China (Chinese)
- 更改国家/语言
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multiparameter flow cytometric analysis of Activated Mac-1 (CD11b) expression on human peripheral blood leucocytes. Phorbol 12-Myristate 13-Acetate (PMA)-stimulated human blood cells were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438, Left Plot) or BD Horizon BV421 Mouse Anti-Human Activated Mac-1 (CD11b) antibody (Cat. No. 566313/566314; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of Activated Mac-1 (CD11b) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
BD Horizon™ BV421 Mouse Anti-Human Activated Mac-1 (CD11b)
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The CBRM1/5 monoclonal antibody specifically recognizes an activated form of the CD11b molecule that is expressed on activated monocytes, granulocytes, NK cells, and subsets of T and B cells. CD11b is also known as the alpha chain of either Mac-1 (MAC-1A/Mac-1α) or Complement receptor 3 (CR3A). CD11b is a ~165 kDa type I transmembrane glycoprotein that is encoded by ITGAM (Integrin alpha M). CD11b associates with CD18 (Integrin beta 2) to form the CD11b/CD18 complex, which is also known as the Mac-1 integrin or the Complement receptor 3 (CR3). CD11b functions in cell-cell and cell-substrate interactions involved in leucocyte activation, adhesion, migration, phagocytosis, and other effector functions. CD11b/CD18 serves as a receptor for multiple ligands including iC3b, CD50 (ICAM-3), CD54 (ICAM-1), CD102 (ICAM-2), and fibrinogen. The CBRM1/5 reportedly inhibits Mac-1-dependent adhesion of stimulated neutrophils to CD54 or fibrinogen.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
研发参考 (4)
-
Diamond MS, Garcia-Aguilar J, Bickford JK, Corbi AL, Springer TA. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.. J Cell Biol. 1993; 120(4):1031-43. (Biology). 查看参考
-
Diamond MS, Springer TA. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen.. J Cell Biol. 1993; 120(2):545-56. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Radioimmunoassay). 查看参考
-
Koleva RI, Ficarro SB, Radomska HS, et al. C/EBPα and DEK coordinately regulate myeloid differentiation.. Blood. 2012; 119(21):4878-88. (Clone-specific: Flow cytometry). 查看参考
-
Müller-Edenborn B, Frick R, Piegeler T, et al. Volatile anaesthetics reduce neutrophil inflammatory response by interfering with CXC receptor-2 signalling.. Br J Anaesth. 2015; 114(1):143-9. (Clone-specific: Flow cytometry). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.