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BUV615 Mouse Anti-Human EphA2
商品详情
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BD OptiBuild™
EPHA2; ECK; ARCC2; CTPA; CTPP1; CTRCT6
Human (Tested in Development)
Mouse IgG2a, κ
Human EphA2 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2875080
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推荐的实验流程

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).

商品通知

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Researchers should determine the optimal concentration of this reagent for their individual applications.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. CF™ is a trademark of Biotium, Inc.
  10. BD Horizon Brilliant Ultraviolet 615 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
751040 Rev. 2
抗体详情
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371805

The 371805 monoclonal antibody specifically recognizes Ephrin type-A receptor 2 (EphA2).  EphA2 is a ~130 kDa type I transmembrane glycoprotein that is encoded by EPHA2 (EPH receptor A2) which belongs to ephrin receptor subfamily within the receptor tyrosine kinase (RTK) family. EphA2 is also known as Epithelial cell kinase (ECK) or Epithelial cell receptor protein tyrosine kinase. The extracellular region of EphA2 contains an N-terminal globular domain, a cysteine-rich domain and 2 fibronectin type III repeats, whereas the cytoplasmic domain contains two tyrosine residues adjacent to the membrane that function as major autophosphorylation sites and a kinase domain. EphA2 is variably expressed by epithelial cells, dendritic cells, Langerhans cells, keratinocytes, and endothelial cells. EphA2 functions as a receptor for glycophosphatidylinositol (GPI) membrane-linked members of the Ephrin-A family, including Ephrins A1-A5. EphA2 is involved in regulating cellular growth, adhesion, migration, survival, and plays a role in angiogenesis. Its expression may be upregulated on vascular endothelium in certain breast, prostate, and colon cancers as well as on some metastatic tumor cells.

The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP.  Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).

751040 Rev. 2
格式详情
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BUV615
The BD Horizon Brilliant™ Ultraviolet 615 (BUV615) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. BUV615, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 615-nm (e.g, 610/20 bandpass filter). The acceptor dye can be excited by the Blue (488-nm) and yellow-green (561-nm) lasers resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV615
Ultraviolet 355 nm
350 nm
615 nm
751040 Rev.2
报价单和参考
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View product citations for antibody "751040" on CiteAb

研发参考 (7)

  1. Brantley DM, Cheng N, Thompson EJ, et al. Soluble Eph A receptors inhibit tumor angiogenesis and progression in vivo. Oncogene. 2002; 21(46):7011. (Biology). 查看参考
  2. Cheng N, Brantley DM, Liu H, et al. Blockade of EphA receptor tyrosine kinase activation inhibits vascular endothelial cell growth factor-induced angiogenesis. Mol Cancer Res. 2002; 1(1):2-11. (Biology). 查看参考
  3. Liao-Chan S, Daine-Matsuoka B, Heald N, et al. Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores.. PLoS ONE. 2015; 10(4):e0124708. (Clone-specific: Bioassay). 查看参考
  4. Lindberg RA, Hunter T. cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases.. Mol Cell Biol. 1990; 10(12):6316-24. (Biology). 查看参考
  5. Udayakumar D, Zhang G, Ji Z, Njauw CN, Mroz P, Tsao H. EphA2 is a critical oncogene in melanoma. Oncogene. 2011; 30(50):4921-4929. (Biology). 查看参考
  6. Udyavar AR, Wooten DJ, Hoeksema M, et al. Novel Hybrid Phenotype Revealed in Small Cell Lung Cancer by a Transcription Factor Network Model That Can Explain Tumor Heterogeneity.. Cancer Res. 2017; 77(5):1063-1074. (Clone-specific: Flow cytometry). 查看参考
  7. de Saint-Vis B, Bouchet C, Gautier G, Valladeau J, Caux C, Garrone P. Human dendritic cells express neuronal Eph receptor tyrosine kinases: role of EphA2 in regulating adhesion to fibronectin. Blood. 2003; 102(13):4431-4440. (Biology). 查看参考
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751040 Rev. 2

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.