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BUV395 Rat Anti-Human TSPAN8
商品详情
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BD OptiBuild™
TM4SF3; transmembrane 4 superfamily member 3; tetraspanin-8; tspan-8; CO-029
Human (Tested in Development)
Rat IgG2b, κ
Human TSPAN8 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872656
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

推荐的实验流程

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

商品通知

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
748225 Rev. 2
抗体详情
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458811

The 458811 monoclonal antibody specifically recognizes Tetraspanin-8 (TSPAN8) that belongs to the Transmembrane 4 (Tetraspanin) superfamily (TM4SF). This molecule is also known as Transmembrane 4 superfamily member 3 (TM4SF3) or Tumor-associated antigen CO-029 (CO-029). TSPAN8 is a ~27-34 kDa protein that spans the membrane four times with transmembrane segments and has intracellular N- and C-termini. TSPAN8 has a small and a large extracellular loop that can mediate dimerization and association with other molecules including integrins, proteases, or cell signaling receptors. TSPAN8 is expressed on hematopoietic progenitor cells, non-keratinized squamous epithelium, endothelial cells, smooth and skeletal muscle cells as well as multiple tumor cell types.  TSPAN8 is involved in angiogenesis, thrombosis, epithelial wound healing and may contribute to tumor cell metastasis.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

748225 Rev. 2
格式详情
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BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV395
Ultraviolet 355 nm
348 nm
395 nm
748225 Rev.2
报价单和参考
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View product citations for antibody "748225" on CiteAb

研发参考 (3)

  1. Jakobsen KR, Paulsen BS, Baek R, Varming K, Sorensen BS, Jorgensen MM. Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma. J Extracell Vesicles. 2015; 4:26659. (Clone-specific: Array, Immunofluorescence). 查看参考
  2. Sondergaard EKL, Pugholm LH, Baek R, Jorgensen MM, Revenfeld ALS, Varming K. Oxygen-Related Differences in Cellular and Vesicular Phenotypes Observed for Ovarian Cell Cancer Lines. J Circ Biomark. 2016; 5:1. (Clone-specific: Array, Immunofluorescence). 查看参考
  3. Yue S, Zhao K, Erb U, Rana S, Zoller M. Joint features and complementarities of Tspan8 and CD151 revealed in knockdown and knockout models. Biochem Soc Trans. 2017; 45(2):437-447. (Biology). 查看参考
748225 Rev. 2

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.