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BUV395 Rabbit Anti- Active Caspase-3
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Consider alternate product [570180], clone C92-605.rMAb, which is manufactured with recombinant technology.
BUV395 Rabbit Anti- Active Caspase-3
Flow cytometric analysis of Active Caspase-3 expressed by apoptotic Jurkat cells. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152) cell line were untreated (dashed line histogram) or treated (solid line histogram) with 4 μM camptothecin for 4 hr to induce apoptosis. The cells were washed once in Dulbecco's PBS, fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714; 20 min at room RT), pelleted and washed with BD Perm/Wash™ Buffer (a component of the kit). The cells were subsequently stained with the BD Horizon™ BUV395 Rabbit Anti-Active Caspase-3 antibody (Cat. No. 564095). The cells were then washed, resuspended in BD Perm/Wash™ Buffer, and analyzed using a BD™ LSR II Flow Cytometer System. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells.
Flow cytometric analysis of Active Caspase-3 expressed by apoptotic Jurkat cells. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152) cell line were untreated (dashed line histogram) or treated (solid line histogram) with 4 μM camptothecin for 4 hr to induce apoptosis. The cells were washed once in Dulbecco's PBS, fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714; 20 min at room RT), pelleted and washed with BD Perm/Wash™ Buffer (a component of the kit). The cells were subsequently stained with the BD Horizon™ BUV395 Rabbit Anti-Active Caspase-3 antibody (Cat. No. 564095). The cells were then washed, resuspended in BD Perm/Wash™ Buffer, and analyzed using a BD™ LSR II Flow Cytometer System. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells.
商品详情
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BD Horizon™
CPP32; Yama; Apopain
Human (QC Testing), Mouse (Tested in Development)
Rabbit IgG
Human Active Caspase-3 Fragment
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2738588
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564095 Rev. 1
抗体详情
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C92-605

The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP).  This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells.  It has not been reported to recognize the pro-enzyme form of caspase-3.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

564095 Rev. 1
格式详情
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BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV395
Ultraviolet 355 nm
348 nm
395 nm
564095 Rev.1
报价单和参考
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View product citations for antibody "564095" on CiteAb

研发参考 (6)

  1. Dai C, Krantz SB. Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells. Blood. 1999; 93(10):3309-3316. (Biology). 查看参考
  2. Dukers DF, Oudejans JJ, Vos W, ten Berge RL, Meijer CJ. Apoptosis in B-cell lymphomas and reactive lymphoid tissues always involves activation of caspase 3 as determined by a new in situ detection method. J Pathol. 2002; 196(3):307-315. (Clone-specific: Immunohistochemistry, Immunoprecipitation). 查看参考
  3. Fujita N, Tsuruo T. Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis. Biochem Biophys Res Commun. 1998; 246(2):484-488. (Biology). 查看参考
  4. Ohsawa S, Hamada S, Yoshida H, Miura M. Caspase-mediated changes in histone H1 in early apoptosis: prolonged caspase activation in developing olfactory sensory neurons. Cell Death Differ. 2008; 15(9):1429-1439. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). 查看参考
  5. Pettersen RD, Bernard G, Olafsen MK, Pourtein M, Lie SO. CD99 signals caspase-independent T cell death. J Immunol. 2001; 166(8):4931-4942. (Clone-specific: Flow cytometry). 查看参考
  6. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316. (Biology). 查看参考
查看所有文件 (6) 查看更少内容
564095 Rev. 1

 

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