Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      Biotin Rat Anti-Mouse CD71
      Biotin Rat Anti-Mouse CD71
      Two-color analysis of the expression of  CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with PE-conjugated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553673) and biotinylated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Biotin Rat Anti-Mouse CD71
      Two-color analysis of the expression of  CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with PE-conjugated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553673) and biotinylated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
      Mouse (QC Testing)
      Rat WF, also known as Wistar Furth IgG1, κ
      Mouse cell line
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      22042
      AB_396690
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      557416 Rev. 10
      抗体详情
      Down Arrow Up Arrow
      C2

      The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.

      557416 Rev. 10
      格式详情
      Down Arrow Up Arrow
      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      557416 Rev.10
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "557416" on CiteAb

      研发参考 (4)

      1. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation). 查看参考
      2. Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation). 查看参考
      3. Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology). 查看参考
      4. Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation). 查看参考
      查看所有文件 (4) 查看更少内容
      557416 Rev. 10

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.