Skip to main content Skip to navigation
Biotin Mouse Anti-Mouse IgG2a[a]
Down Arrow Up Arrow

BD Pharmingen™
Mouse (QC Testing)
Mouse SJL IgG2a, κ
Not reported
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.


The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.


Biotinylated 8.3 mAb may be used as a secondary reagent in immunofluorescent staining with flow cytometric analysis. Note: The isotype of clone 8.3  is mouse (SJL) IgG2a[b], κ.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553502 Rev. 17
Down Arrow Up Arrow

The 8.3 antibody reacts specifically with mouse IgG2a of Igh-Ca and the d, e, f, g, h, j, n, and o haplotypes (eg, BALB/c, C58, and A, AKR, CBA, C3H/He, NZB). It does not react with IgG2a of Igh-Cb nor the c and p haplotypes (eg, C57BL/6, C57BL/10, SJL, DBA/1, DBA/2, SWR).

553502 Rev. 17
Down Arrow Up Arrow
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553502 Rev.17
Down Arrow Up Arrow
View product citations for antibody "553502" on CiteAb

研发参考 (2)

  1. Huang CM, Parsons M, Oi VT, Huang HJ, Herzenberg LA. Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):311-321. (Clone-specific). 查看参考
  2. Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Clone-specific). 查看参考
553502 Rev. 17

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.