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Biotin Hamster Anti-Mouse CD3e
Biotin Hamster Anti-Mouse CD3e
Flow cytometric analysis of CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048), PE-Rat Anti-Mouse CD8a (Cat. No. 553032) and Biotin Hamster Anti-Mouse CD3e (Cat. No. 553060/553059; bottom left panel), followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes were also stained with Biotin Hamster Anti-Mouse CD3e, followed by Avidin FITC (Cat. No. 553059/553060; bottom right panel). Fluorescence contour plots and histograms were derived from gated events based on the forward and side light-scattering of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048), PE-Rat Anti-Mouse CD8a (Cat. No. 553032) and Biotin Hamster Anti-Mouse CD3e (Cat. No. 553060/553059; bottom left panel), followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes were also stained with Biotin Hamster Anti-Mouse CD3e, followed by Avidin FITC (Cat. No. 553059/553060; bottom right panel). Fluorescence contour plots and histograms were derived from gated events based on the forward and side light-scattering of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.
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BD Pharmingen™
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Mouse (QC Testing)
Armenian Hamster IgG1, κ
H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported)
0.5 mg/ml
12501
AB_394593
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
553060 Rev. 20
抗体详情
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145-2C11

The 145-2C11 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex that is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events that activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells.  In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes. Preincubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining reportedly enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes.

553060 Rev. 20
格式详情
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553060 Rev.20
报价单和参考
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View product citations for antibody "553060" on CiteAb

研发参考 (9)

  1. Duke RC, Cohen JJ, Boehme SA, et al. Morphological, biochemical, and flow cytometric assays of apoptosis. In: Coligan J, Kruisbeek AM, Margulies D, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:3.17.1-3.17.33.
  2. Isakov N, Wange RL, Burgess WH, Watts JD, Aebersold R, Samelson LE. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J Exp Med. 1995; 181(1):375-380. (Biology: Immunoprecipitation). 查看参考
  3. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Methodology: Activation, Stimulation). 查看参考
  4. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Biology). 查看参考
  5. Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A. 1987; 84(5):1374-1378. (Immunogen: Activation, Blocking, Cytotoxicity, Immunoprecipitation, Stimulation). 查看参考
  6. Nakano H, Yamazaki T, Miyatake S, Nozaki N, Kikuchi A, Saito T. Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex. J Biol Chem. 1996; 271(11):6483-6489. (Biology: Immunoprecipitation). 查看参考
  7. Portoles P, Rojo J, Golby A, et al . Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989; 142(12):4169-4175. (Biology: Activation, Immunoprecipitation, Stimulation). 查看参考
  8. Shinkai Y, Alt FW. CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression. Int Immunol. 1994; 6(7):995-1001. (Biology: Activation, Stimulation). 查看参考
  9. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996; 184(3):1083-1091. (Biology: Immunohistochemistry). 查看参考
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553060 Rev. 20

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