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Biotin Goat Anti-Rat Ig

BD Pharmingen™ Biotin Goat Anti-Rat Ig

克隆 Polyclonal

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BD Pharmingen™
Rat (QC Testing)
Goat Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
554014 Rev. 20
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This polyclonal antibody reacts with whole molecule rat IgG (heavy and light chains) and may also react with the light chains of other rat immunoglobulins.  The antibody has been reported to be reactive in ELISA with rat IgG, IgM, and IgA.  In addition, some cross-reactivity with mouse IgG, some hamster Ig and minimal cross-reactivity with human Ig has been reported.   Investigators experiencing cross-reactivity with rat IgM may wish to consider using the following alternatives:  Biotin Mouse Anti-Rat IgM (Cat. No. 553886) or Biotin Mouse Anti-Rat Ig, κ light chain (Cat. No. 553871).  Minimal cross-reaction to mouse, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected.  

For immunohistochemical staining (IHC) of acetone-fixed frozen and paraffin-embedded sections, Biotin Goat Anti-Rat Ig (Cat. No. 559286) is recommended.  When using rat IgG2b primary antibodies, Biotin Mouse Anti-Rat IgG2b (Cat. No. 550327) is also available as an alternate. When using rat IgM primary antibodies, Mouse Anti-Rat/Hamster Ig κ light chain (Cat. No. 550336) or Mouse Anti-Rat IgM (Cat. No. 550330) are also available alternatives.

554014 Rev. 20
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
554014 Rev.20
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554014 Rev. 20

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.