Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      APC Mouse Anti-Human CD195
      APC Mouse Anti-Human CD195
      Flow cytometric analysis of CD195 expression on human blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD195 (Cat. No. 556903/561748; solid line histogram) or APC Mouse IgG2a, κ Isotype Control (Cat. No. 555576; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms depicting CD195 (or Ig isotype control) expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
      APC Mouse Anti-Human CD195
      Flow cytometric analysis of CD195 expression on human blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD195 (Cat. No. 556903/561748; solid line histogram) or APC Mouse IgG2a, κ Isotype Control (Cat. No. 555576; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms depicting CD195 (or Ig isotype control) expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      CCR5; C-C chemokine receptor type 5; CC-CKR-5; CKR5; CHEMR13
      Human (QC Testing)
      Mouse C57BL/6 IgG2a, κ
      Human CCR5 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      20 µl
      VII 70307
      1234
      AB_398619
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      推荐的实验流程

      Immunophenotyping studies of chemokine receptors need to be performed on freshly collected whole blood (<24 hours). Incubation with the antibody should be done at room temperature in the dark. Cellular manipulation, such as Ficoll-Paque™ separation, freezing, or exposure to cold temperatures prior to staining have been shown to cause a decrease in staining intensity and inconsistent results.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
      7. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      561748 Rev. 2
      抗体详情
      Down Arrow Up Arrow
      2D7/CCR5

      The 2D7/CCR5 monoclonal antibody specifically binds to the human chemokine receptor, CCR5. CCR5 is also known as CD195. CCR5 is a seven transmembrane-spanning G protein-associated molecule. CCR5 belongs to the beta chemokine receptor family. It is expressed on a subset of T lymphocytes (CD3+CD45RO+CD95+). CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1), and monocyte chemoattractant protein 2 (MCP-2).  Additionally, CCR5 has been found to be a co-receptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission. Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection. This antibody has been shown to block ligand and gp120 binding. It is also able to neutralize HIV infection.

      561748 Rev. 2
      格式详情
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      561748 Rev.2
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (8)

      1. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996; 85(7):1135-1148. (Biology). 查看参考
      2. Deng H, Liu R, Ellmeier W, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 1996; 381(6584):661-666. (Biology: Calcium Flux). 查看参考
      3. Doranz BJ, Rucker J, Yi Y, et al. A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors. Cell. 1996; 85(7):1149-1158. (Biology). 查看参考
      4. Dragic T, Litwin V, Allaway GP, et al. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 1996; 381(6584):667-673. (Biology). 查看参考
      5. Hancock WW. Chemokines and the pathogenesis of T cell-dependent immune responses. Am J Pathol. 1996; 148(3):681-684. (Biology). 查看参考
      6. Ponath P. Leukosite, Inc. . .
      7. Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. J Biol Chem. 1996; 271(29):17161-17166. (Biology). 查看参考
      8. Wu L, Paxton WA, Kassam N, et al. CCR5 levels and expression pattern correlate with infectability by macrophage-tropic HIV-1, in vitro. J Exp Med. 1997; 185(9):1681-1689. (Biology). 查看参考
      查看所有文件 (8) 查看更少内容
      561748 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback