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Functional Assays
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Flow cytometric analysis of Mouse IL-23 p19 expression. 293-F cells, either transfected with mouse IL-23 p19 (solid line histograms) or untransfected (dotted line histograms), were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714) before staining with either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731) or Alexa Fluor® 647 Rat Anti-Mouse IL-23 p19 (Cat. No. 565317) at 0.25 µg per test. The histograms show the expression of IL-23 p19 (Right plot) or isotype control staining (Left plot) on gated events with the forward and side light-scatter characteristics of the intact cell population. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse IL-23 p19
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The N71-1183 monoclonal antibody specifically recognizes the alpha subunit, also known as p19, of mouse interleukin 23 that is encoded by the Il23a gene. Interleukin 23 (IL-23) is a heterodimeric cytokine composed of a p40 subunit and a p19 subunit. The p19 subunit is unique to IL-23, while the p40 subunit is shared with IL-12 and is also known as IL-12B. Human and mouse p19 share 70% amino acid sequence identity. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4 and TLR8 ligands, as well as the β-glucan receptor, Dectin-1. IL-23 has biological activities that are similar to, but distinct from IL-12: Both IL12 and IL-23 induce proliferation and IFN-γ production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 are restricted to memory T cells. IL-23, but not IL-12, has been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. In vitro and in vivo treatment with N71-1183 monoclonal antibody has been shown to inhibit IL-23's ability to enhance IL-17 production by activated MRL/lpr T lymphocytes (Kyttaris VC, Kampagianni O, Tsokos GC, 2013).
研发参考 (2)
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Kyttaris VC, Kampagianni O, Tsokos GC. Treatment with Anti-Interleukin 23 Antibody Ameliorates Disease in Lupus-Prone Mice.. Biomed Res Int. 2013; 2013Available: http://www.ncbi.nlm.nih.gov/pubmed/23841097 2014, December 3. 查看参考
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Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Biology). 查看参考
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