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Alexa Fluor® 647 Rat Anti-Mouse CD73
Alexa Fluor® 647 Rat Anti-Mouse CD73

Multicolor flow cytometric analysis for CD73 expression on mouse spleen cells. C57BL/6 mouse spleen cells were stained with PE hamster anti-mouse CD3e (Cat. No. 553064/553063) and with either an Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat No. 557690, Left Panel) or with the Alexa Fluor® 647 Rat Anti-Mouse CD73 antibody (Cat No. 561543, Right Panel). Two-color flow cytometric dot plots show the expression of CD73 (or Ig isotype control staining) versus CD3e and were derived from events with the forward and side light-scatter characteristics of viable spleen cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis for CD73 expression on mouse spleen cells. C57BL/6 mouse spleen cells were stained with PE hamster anti-mouse CD3e (Cat. No. 553064/553063) and with either an Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat No. 557690, Left Panel) or with the Alexa Fluor® 647 Rat Anti-Mouse CD73 antibody (Cat No. 561543, Right Panel). Two-color flow cytometric dot plots show the expression of CD73 (or Ig isotype control staining) versus CD3e and were derived from events with the forward and side light-scatter characteristics of viable spleen cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

商品详情
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BD Pharmingen™
Ecto-5'-nucleotidase; 5'-NT; 5NTD; eNT; NT; Nt5; Nt5e; Nte
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
BALB/c mouse splenocytes and CHO cells transfected with the Mouse CD73 gene, Nt5
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10896329
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561543 Rev. 1
抗体详情
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TY/23

The TY/23 monoclonal antibody specifically binds to CD73 or Ecto-5'-nucleotidase (5'-NT), a 69 kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. CD73 expression appears to be developmentally regulated on leukocytes. In the bone marrow, it is found on most CD11b+ myeloid cells and very few CD19+ cells of the B-lymphocyte lineage. It is not found on CD11b+ cells in the periphery nor on marrow-derived GMCSF-stimulated dendritic cells. Some peripheral B lymphocytes express CD73, with higher levels on isotype-switched B cells. The few thymocytes which have detectable surface CD73 are of the CD4-CD8- (double negative) and the single-positive populations. In the peripheral lymphoid organs, significant subpopulations of the CD4+ and CD8+ T lymphocytes express CD73, with variation in the percentages of CD73-bearing T cells observed among inbred mouse strains. In the thymus and peripheral lymphoid organs, CD73 is found on endothelia and stromal cells. CD73 has also been detected on bone marrow and thymic epithelial cell lines, kidney glomeruli and proximal-tubule epithelial cells, and liver endothelial cells and hepatocytes. The TY/23 mAb inhibits the enzymatic activity of CD73. Although soluble TY/23 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with PMA, but not with Concanavalin-A or plate-bound anti-CD3ε mAb 145-2C11.

561543 Rev. 1
格式详情
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
561543 Rev.1
报价单和参考
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研发参考 (2)

  1. Resta R, Yamashita Y, Thompson LF. Ecto-enzyme and signaling functions of lymphocyte CD73. Immunol Rev. 1998; 161:95-109. (Biology). 查看参考
  2. Yamashita Y, Hooker SW, Jiang H, et al. CD73 expression and fyn-dependent signaling on murine lymphocytes. Eur J Immunol. 1998; 28(10):2981-2990. (Immunogen). 查看参考
561543 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.