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      Alexa Fluor® 647 Rat Anti-Human B7-H6
      Alexa Fluor® 647 Rat Anti-Human B7-H6
      Flow cytometric analysis of B7-H6 expression on B lymphoblasts from Burkitt's lymphoma. RAJI cells (ATCC® CCL-86) were stained with either Alexa Fluor® 647 Rat IgM, κ Isotype Control (Cat.No. 560892; dashed line) or Alexa Fluor® 647 Rat Anti-Human B7-H6 (Cat. No. 566733; solid line) at 0.25ug/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor® 647 Rat Anti-Human B7-H6
      Flow cytometric analysis of B7-H6 expression on B lymphoblasts from Burkitt's lymphoma. RAJI cells (ATCC® CCL-86) were stained with either Alexa Fluor® 647 Rat IgM, κ Isotype Control (Cat.No. 560892; dashed line) or Alexa Fluor® 647 Rat Anti-Human B7-H6 (Cat. No. 566733; solid line) at 0.25ug/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Pharmingen™
      B7 homolog 6; B7-H6; B7H6; DKFZp686O24166; NCR3LG1
      Human (QC Testing)
      Rat IgM, κ
      Human B7-H6 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2869835
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566733 Rev. 1
      抗体详情
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      1A5

      The 1A5 monoclonal antibody specifically recognizes B7 homolog 6 (B7-H6). B7-H6 is a ~51 kDa type I transmembrane glycoprotein that is encoded by NCR3LG1 (natural killer cell cytotoxicity receptor 3 ligand 1) which belongs to the B7 family of immunoreceptors. B7-H6 contains one IgV-like and one IgC1-like domain in its extracellular region followed by a transmembrane region and an intracytoplasmic region with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an SH2- and SH3-binding motifs. Although B7-H6 is not expressed by normal cells from healthy individuals, it is variably expressed on stressed cells including tumor cells and tumor cell lines, and can be moderately expressed on proinflammatory monocytes and neutrophils. Binding of B7-H6 to CD337 (NKp30/NCR3) induces NK cell activation, cytotoxicity, and cytokine secretion which suggests it may play an important role in immunosurveillance against certain tumors and infectious diseases.

      566733 Rev. 1
      格式详情
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566733 Rev.1
      报价单和参考
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      View product citations for antibody "566733" on CiteAb

      研发参考 (3)

      1. Brandt CS, Baratin M, Yi EC, et al. The B7 family member B7-H6 is a tumor cell ligand for the activating natural killer cell receptor NKp30 in humans. J Exp Med. 2009; 206(7):1495-1503. (Biology). 查看参考
      2. Matta J, Baratin M, Chiche L, et al. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions. Blood. 2013; 122(3):394-404. (Biology). 查看参考
      3. Wang R, Jaw JJ, Stutzman NC, Zou Z, Sun PD. Natural killer cell-produced IFN-γ and TNF-α induce target cell cytolysis through up-regulation of ICAM-1.. J Leukoc Biol. 2012; 91(2):299-309. (Clone-specific: Flow cytometry). 查看参考
      566733 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.