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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
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- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Two-color flow cytometric analysis of CRTAM (CD355) expression on activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours with Phorbol 12-Myristate 13-Acetate (PMA; 10 ng/ml) and Ionomycin (1 µg/ml). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD8 antibody (Cat.No. 562428) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355) antibody (Cat. No. 566645; Right Plot) at 1 µg/test. Two-color flow-cytometric pseudocolor plots showing the correlated expression of CD8 versus CRTAM (CD355) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355)
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
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配套商品
The CR24.1 monoclonal antibody specifically recognizes Class I MHC-restricted T cell-associated molecule (CRTAM) which is also known as CD355, Class I-restricted T cell-associated molecule, or Cytotoxic and regulatory T-cell molecule. CRTAM (CD355) is a type I transmembrane glycoprotein that belongs to the Nectin family of cell adhesion molecules within the Ig superfamily. It is expressed on activated NK cells, NKT cells, γδ T cells, CD8+ T cells, and by Purkinje neurons of the cerebellum. CRTAM can exist as a homodimer or as a heterodimer when bound to its ligand, Nectin-like molecule 2 (Necl-2), which is encoded by TSLC1 (tumor suppressor gene tumor suppressor in lung cancer-1). Necl-2-CRTAM interactions play important roles in cellular adhesion and migration and can augment IFN-γ production by CD8+ T cells and cytotoxicity by NK cells.
研发参考 (5)
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Boles KS, Barchet W, Diacovo T, Cella M, Colonna M. The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM.. Blood. 2005; 106(3):779-86. (Immunogen: Blocking, Flow cytometry). 查看参考
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Dessarthe B, Thedrez A, Latouche JB, et al. CRTAM receptor engagement by Necl-2 on tumor cells triggers cell death of activated Vγ9Vδ2 T cells.. J Immunol. 2013; 190(9):4868-76. (Biology). 查看参考
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Galibert L, Diemer GS, Liu Z, et al. Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule.. J Biol Chem. 2005; 280(23):21955-64. (Biology). 查看参考
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Kennedy J, Vicari AP, Saylor V, et al. A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM).. J Leukoc Biol. 2000; 67(5):725-34. (Biology). 查看参考
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Patiño-Lopez G, Hevezi P, Lee J, et al. Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes.. J Neuroimmunol. 2006; 171(1-2):145-55. (Biology). 查看参考
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