-
Reagents
- Flow Cytometry Reagents
-
蛋白质印迹试剂
- 免疫分析 试剂
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
-
Functional Assays
-
显微成像试剂
-
Cell Preparation and Separation Reagents
-
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- China (Chinese)
- 更改国家/语言
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multiparameter flow cytometric analysis of CLM-8 (CD300a) expression on human peripheral blood leucocyte populations. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CLM-8 (CD300a) antibody (Cat. No. 566342; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of CLM-8 (CD300a) [or Ig Isotype control staining] versus side light-scatter signals were derived from gated events with the forward and side light-scattering characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CLM-8 (CD300a)
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The MEM-260 monoclonal antibody specifically recognizes CD300a which is also known as CMRF35-like molecule 8 (CLM8), Inhibitory receptor protein 60 (IRp60), or Immunoglobulin superfamily member 12 (IGSF12). CD300a is a ~60 kDa type I transmembrane glycoprotein that belongs to the CMRF family within the Ig gene superfamily. CD300a is expressed on natural killer (NK) cells, T and B cell subsets, monocytes, macrophages, dendritic cells, granulocytes and mast cells. CD300a has a single Ig V-like extracellular domain which is homologous to the Ig-binding domains of the of the Fc receptor for polymeric IgA and IgM. CD300a is a non-MHC-specific inhibitory receptor that contains three immunoreceptor tyrosine-based inhibitory motifs in its intracellular region. CD300a can reportedly inhibit NK cell cytolytic activity and granulocyte and mast cell functions.
研发参考 (7)
-
Alvarez Y, Tang X, Coligan JE, Borrego F. The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling.. Mol Immunol. 2008; 45(1):253-8. (Biology). 查看参考
-
Borrego F. The CD300 molecules: an emerging family of regulators of the immune system. Blood. 2013; 121(11):1951-1960. (Biology). 查看参考
-
DeBell KE, Simhadri VR, Mariano JL, Borrego F. Functional requirements for inhibitory signal transmission by the immunomodulatory receptor CD300a.. BMC Immunol. 2012; 13:23. (Biology). 查看参考
-
Kim EJ, Lee SM, Suk K, Lee WH. CD300a and CD300f differentially regulate the MyD88 and TRIF-mediated TLR signalling pathways through activation of SHP-1 and/or SHP-2 in human monocytic cell lines.. Immunology. 2012; 135(3):226-35. (Clone-specific: Functional assay). 查看参考
-
Lankry D, Rovis TL, Jonjic S, Mandelboim O. The interaction between CD300a and phosphatidylserine inhibits tumor cell killing by NK cells.. Eur J Immunol. 2013; 43(8):2151-61. (Biology). 查看参考
-
Sabato V, Verweij MM, Bridts CH, et al. CD300a is expressed on human basophils and seems to inhibit IgE/FcεRI-dependent anaphylactic degranulation.. Cytometry B Clin Cytom. 2012; 82(3):132-8. (Clone-specific: Flow cytometry, Functional assay). 查看参考
-
Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.