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Multiparameter flow cytometric analysis of CD307d (FCRL4) expression in resting and activated B lymphocytes. Peripheral blood mononuclear cells were cultured overnight without (left column) or with (right column) Anti-Human IgM F(ab')2 and CpG ODN2006. The cells were harvested, incubated with Human BD Fc Block™ (Cat. No. 564220), and then stained with PE Mouse Anti-Human CD19 (Cat. No. 555413 or 561741) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357, top row) or Alexa Fluor® 647 Mouse Anti-Human CD307d (FCRL4) (Cat. No. 566587, bottom row). Two-color contour plots showing the correlated expression of CD307d (FCRL4) [or Ig isotype control staining] versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD307d (FCRL4)
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- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The A1/CD307d monoclonal antibody specifically recognizes CD307d which is also known as Fc receptor-like protein 4 (FCRL4), Fc receptor homolog 4 (FCRH4), or Immune receptor translocation-associated protein 1 (IRTA1). CD307d (FCRL4) is a type I transmembrane glycoprotein that belongs to the IRTA gene family within the Ig gene superfamily. It has four extracellular Ig-like domains, a helical transmembrane region, and two consensus immunoreceptor tyrosine-based inhibition motifs (ITIMs) and one immunoreceptor tyrosine-based switch motif (ITSM) in its cytoplasmic domain. CD307d (FCRL4) binds to aggregated IgA and IgG, and its highest level of expression is on memory B cells in mucosa-associated lymphoid tissues (MALT). Chromosomal abnormalities in the region of the FCRL4 gene are associated with non-Hodgkin lymphoma and multiple myeloma, and CD307d (FCRL4) may play a role in HIV-induced memory B cell dysfunction.
研发参考 (9)
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Hatzivassiliou G, Miller I, Takizawa J, et al. IRTA1 and IRTA2, novel immunoglobulin superfamily receptors expressed in B cells and involved in chromosome 1q21 abnormalities in B cell malignancy. Immunity. 2001; 14(3):277-289. (Biology). 查看参考
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Jelicic K, Cimbro R, Nawaz F, et al. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression.. Nat Immunol. 2013; 14(12):1256-65. (Biology). 查看参考
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Lestou VS, Ludkovski O, Connors JM, Gascoyne RD, Lam WL, Horsman DE. Characterization of the recurrent translocation t(1;1)(p36.3;q21.1-2) in non-Hodgkin lymphoma by multicolor banding and fluorescence in situ hybridization analysis.. Genes Chromosomes Cancer. 2003; 36(4):375-81. (Biology). 查看参考
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Li FJ, Won WJ, Becker EJ, et al. Emerging roles for the FCRL family members in lymphocyte biology and disease.. Curr Top Microbiol Immunol. 2014; 382:29-50. (Biology). 查看参考
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Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol Lett. 2011; 134(2):113-121. (Clone-specific: Flow cytometry). 查看参考
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Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P. New B-cell CD molecules. Immunol Lett. 2011; 134(2):104-112. (Clone-specific: Flow cytometry). 查看参考
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Psathas JN, Doonan PJ, Raman P, Freedman BD, Minn AJ, Thomas-Tikhonenko A. The Myc-miR-17-92 axis amplifies B-cell receptor signaling via inhibition of ITIM proteins: a novel lymphomagenic feed-forward loop.. Blood. 2013; 122(26):4220-9. (Biology). 查看参考
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Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012; 188(10):4741-4745. (Biology). 查看参考
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