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BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD3
克隆 MEM-57 (RUO)
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD3 antibody (Cat. No. 566334; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter dot plots showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
商品详情
BD Pharmingen™
CD3-epsilon; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE
Human (QC Testing)
Mouse IgG2a
Human Thymocytes and T Lymphocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
IV T96 (T096)
AB_2739691
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 ML
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 ML
品类编号
554657
Lysing Buffer RUO
尺寸
100 ML
品类编号
555899
Lysing Solution 10X Concentrate CFDA_IVD
尺寸
100 ML
品类编号
349202
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
尺寸
100 Tests
品类编号
557715
566334 Rev. 1
抗体详情
MEM-57
The human CD3 complex consists of four transmembrane proteins (γ, δ, ε, ζ) that are associated with the T cell antigen receptor (TCR) to form the CD3/TCR complex. The CD3 complex associates with either TCR αβ or TCR γδ heterodimers that are expressed by some thymocytes, T cells or NKT cells. The CD3 complex is required for the cell surface expression and signal-transducing functions of the TCR. The CD3 complex is expressed by ~60-85% thymocytes and ~60-80% of normal human peripheral blood lymphocytes. The MEM-57 monoclonal antibody reportedly recognizes the human CD3 epsilon subunit (CD3e/CD3ε, also known as T3E or TCRE) of the CD3/TCR complex. CD3e is a ~20 kDa unglycosylated transmembrane protein that belongs to the Ig gene superfamily. CD3e has an Ig-like extracellular domain (ECD) and an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The MEM-57 antibody reportedly stimulates T cell proliferation and can induce cytotoxicity in some human T cell lines.
566334 Rev. 1
格式详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
653 nm
669 nm
566334 Rev.1
报价单和参考
View product citations for antibody "566334" on CiteAb
研发参考 (5)
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Ernst DN, Shih CC. CD3 complex. J Biol Regul Homeost Agents. 2000; 14(3):226-229. (Biology). 查看参考
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Kurrle R. Cluster report: CD3. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:290-293.
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Pan Q, Brodeur JF, Drbal K, Dave VP. Different role for mouse and human CD3delta/epsilon heterodimer in preT cell receptor (preTCR) function: human CD3delta/epsilon heterodimer restores the defective preTCR function in CD3gamma- and CD3gammadelta-deficient mice.. Mol Immunol. 2006; 43(11):1741-50. (Clone-specific: Flow cytometry, Immunoprecipitation). 查看参考
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Transy C, Moingeon PE, Marshall B, Stebbins C, Reinherz EL. Most anti-human CD3 monoclonal antibodies are directed to the CD3 epsilon subunit.. Eur J Immunol. 1989; 19(5):947-50. (Clone-specific: Flow cytometry). 查看参考
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Tunnacliffe A, Olsson C, Traunecker A, Krissansen GW, Karjalainen K, de la Hera A. The majority of CD3 epitopes are conferred by the epsilon chain. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:295-296.
566334 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.