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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells (dashed line histogram) and human TGF-β1-transfected P3UI cells (solid line histogram) were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722) and washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor® 488 Mouse Anti-Human TGF-β1 antibody (Cat. No. 562545). The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 488 Mouse Anti-Human TGF-β1
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准备和存储
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
配套商品
The TW4-9E7 monoclonal antibody specifically binds to Human Transforming Growth Factor beta-1 (TGF-β1). TGF-β1 is a potent multifunctional cytokine that positively or negatively regulates numerous processes including development, hematopoiesis, tissue remodeling, wound repair, innate and adaptive immunity as well as cancer and autoimmune diseases. TGF-β1 is formed by the enzymatic cleavage of the TGF-β1 propeptide that is encoded by the TGFB1 gene and comprised of the Latency Associated Peptide (LAP) and TGF-β1. Prior to secretion, the dimeric LAP-TGF-β1 propeptide is cleaved resulting in a biologically inactive form of dimeric TGF-β1 that is noncovalently associated with dimeric LAP (latent TGF-β1). This complex may be expressed on the surface of TGF-β1-producing cells or be further processed by proteolytic removal of LAP to release the biologically active mature form of the soluble TGF-β1 homodimer. Many different cell types synthesize TGF-β1 and express specific receptors for it. The TW4-9E7 antibody recognizes both the intracellular latent bound form of TGF-β1 along with the membrane bound form of TGF-β1.
研发参考 (6)
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Derynck R, Akhurst RJ. Differentiation plasticity regulated by TGF-beta family proteins in development and disease. Nat Cell Biol. 2007; 9(9):1000-1004. (Biology). 查看参考
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Derynck R, Jarrett JA, Chen EY, et al. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985; 316(6030):701-705. (Biology). 查看参考
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Dünker N, Krieglstein K. Targeted mutations of transforming growth factor-beta genes reveal important roles in mouse development and adult homeostasis. Eur J Biochem. 2000; 267(24):6982-6988. (Biology). 查看参考
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Miyazono K, Hellman U, Wernstedt C, Heldin CH. Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization. J Biol Chem. 1988; 263(13):6407-6415. (Biology). 查看参考
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Oida T, Weiner HL. Overexpression of TGF-β1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-β. J Immunol. 2010; 185(6):3529-3535. (Immunogen: Flow cytometry). 查看参考
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Rubtsov YP, Rudensky AY. TGFbeta signalling in control of T-cell-mediated self-reactivity. Nat Rev Immunol. 2007; 7(6):443-453. (Biology). 查看参考
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