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      Alexa Fluor® 488 Mouse Anti-Human CD15s
      Alexa Fluor® 488 Mouse Anti-Human CD15s
      Two-parameter flow cytometric analysis of CD15s expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 488 Mouse IgM, κ Isotype Control (Cat. No. 562409; Left Panel) or Alexa Fluor® 488 Mouse Anti-Human CD15s antibody (Cat. No. 563528; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter dot plots show the correlated expression patterns of CD15s (or Ig Isotype control staining) versus side light-scatter characteristics for gated events with the light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Alexa Fluor® 488 Mouse Anti-Human CD15s
      Two-parameter flow cytometric analysis of CD15s expression on human peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 488 Mouse IgM, κ Isotype Control (Cat. No. 562409; Left Panel) or Alexa Fluor® 488 Mouse Anti-Human CD15s antibody (Cat. No. 563528; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter dot plots show the correlated expression patterns of CD15s (or Ig Isotype control staining) versus side light-scatter characteristics for gated events with the light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      商品详情
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      BD Pharmingen™
      Sialyl Lewis X
      Human (QC Testing)
      Mouse BALB/c IgM, κ
      Membrane proteins from human tomach adenocarcinoma tissue
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2738260
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
      7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563528 Rev. 1
      抗体详情
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      CSLEX1

      The CSLEX1 monoclonal antibody specifically binds to CD15s. This anti-Sialyl-Le[x] monoclonal antibody is specific for the α2-3 sialosylated form of lacto-N-fucopentaose III, sialyl Lex (Sle[x]). Sle[x] is expressed on granulocytes, monocytes and both normal and tumor cells of diverse origin. It has been shown to be a ligand for both endothelial leukocyte adhesion molecule-1 (ELAM-1 or E-selectin), and granule membrane protein-140 (GMP-140 or P-selectin).

      563528 Rev. 1
      格式详情
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      Alexa Fluor™ 488
      Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 488
      Blue 488 nm
      494 nm
      517 nm
      563528 Rev.1
      报价单和参考
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      研发参考 (11)

      1. Corral L, Singer MS, Macher BA, Rosen SD. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets. Biochem Biophys Res Commun. 1990; 172(3):1349-1356. (Biology). 查看参考
      2. Dransfield I, Stocks SC, Haslett C. Regulation of cell adhesion molecule expression and function associated with neutrophil apoptosis. Blood. 1995; 85(11):3264-3273. (Clone-specific: Flow cytometry). 查看参考
      3. Fukushima K, Hirota M, Terasaki PI. Characterization of sialosylated Lewisx as a new tumor-associated antigen. Cancer Res. 1984; 44(11):5279-5285. (Immunogen: Cytotoxicity, ELISA, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Radioimmunoassay). 查看参考
      4. Phillips ML, Nudelman E, Gaeta FC, et al. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990; 250(4984):1130-1132. (Biology). 查看参考
      5. Picker LJ, Warnock RA, Burns AR, Doerschuk CM, Berg EL, Butcher EC. The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell. 1991; 66(5):921-933. (Biology). 查看参考
      6. Polley MJ, Phillips ML, Wayner E, et al. CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proc Natl Acad Sci U S A. 1991; 88(14):6224-6228. (Biology). 查看参考
      7. Rice GE, Bevilacqua MP. An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science. 1989; 246(4935):1303-1306. (Biology). 查看参考
      8. Rice GE, Gimbrone MA, Bevilacqua MP. Tumor cell-endothelial interactions. Increased adhesion of human melanoma cells to activated vascular endothelium. Am J Pathol. 1988; 133(2):204-210. (Biology). 查看参考
      9. Walz G, Aruffo A, Kolanus W, Bevilacqua M, Seed B. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. Science. 1990; 250(4984):1132-1135. (Biology). 查看参考
      10. Zetter BR. The cellular basis of site-specific tumor metastasis. N Engl J Med. 1990; 322(9):605-612. (Biology). 查看参考
      11. Zhou Q, Moore KL, Smith DF, Varki A, McEver RP, Cummings RD. The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells. J Cell Biol. 1991; 115(2):557-564. (Clone-specific: ELISA). 查看参考
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      563528 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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