BD Pharmingen™ PE Mouse Anti- BrdU Set
克隆 MOPC-21 (also known as MOPC21; MOPC 21) (RUO)
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System
描述
Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics.
The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU.
准备和存储
推荐的实验流程
Investigators: Please note that this product is routinely tested on fixed and permeabilized REH cell line with 70-80% cold ethanol. Please see the detailed protocol example at http://www.bdbiosciences.com/us/resources/protocols/s/brdudetection This product is NOT recommended to use with the BrdU flow kits (e.g. Cat. Nos. 559619, 552598, 557892 and 557892).
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- An isotype control should be used at the same concentration as the antibody of interest.
| 描述 | 数量/尺寸 | 零件号 | 克隆 | 同型对照 |
|---|---|---|---|---|
| PE Mouse IgG1, κ Isotype Control | 100 Tests (1 ea) | 51-35405X | MOPC-21 | IgG1, κ |
| PE Mouse Anti-BrdU | 100 Tests (1 ea) | 51-33285X | 3D4 | IgG1, κ |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.