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Reagents
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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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BD Pharmingen™ Ig Isotype Control Cocktail - B
(RUO)Ig Isotype Control Cocktail - B
Regulatory Status Legend
未经BD明确书面授权,严禁使用未经许可的任何商品。
描述
The Isotype Control Cocktail B is a mixture of Mouse IgG1, Mouse IgG2a, and Mouse IgM designed and optimized to work in conjunction with the Rat T Lymphocyte Cocktail (Cat. No. 558493). The Mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells. The Mouse IgG2a G155-178 antibody was developed against TNP as well, but has unknown specificity. The Mouse IgG1 MOPC-21 immunoglobulin is a mouse myeloma protein. All immunoglobulins were selected as isotype controls following screening for low background on a variety of mouse and human tissues.
准备和存储
推荐的实验流程
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
描述 | 数量/尺寸 | 零件号 | EntrezGene ID |
---|---|---|---|
APC mIgM | N/A | 51-590-03089 | N/A |
PE Mouse IgG2a, κ Isotype Control | N/A | 51-550-33035 | N/A |
FITC mIgG1 | N/A | 51-540-20604 | N/A |
研发参考 (1)
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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