描述
Cocktail Component Clone
APC mIgM G155-228
PE mIgG2a G155-178
FITC mIgG1 MOPC-21
The Isotype Control Cocktail B is a mixture of Mouse IgG1, Mouse IgG2a, and Mouse IgM designed and optimized to work in conjunction with the Rat T Lymphocyte Cocktail (Cat. No. 558493). The Mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells. The Mouse IgG2a G155-178 antibody was developed against TNP as well, but has unknown specificity. The Mouse IgG1 MOPC-21 immunoglobulin is a mouse myeloma protein. All immunoglobulins were selected as isotype controls following screening for low background on a variety of mouse and human tissues.
准备和存储
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
研发参考 (1)
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
