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Purified Mouse IgM, κ Isotype Control
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BD Pharmingen™
b allotype, anti-KLH
Mouse C57BL/6 IgM, κ
ELISA Standard (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Isotype control (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


C48-6 is useful as a standard in ELISA or as an isotype-matched negative control for immunostaining.  Other reported applications include immunohistochemical staining (IHC) of acetone-fixed frozen and formalin-fixed paraffin-embedded sections.  For IHC, we recommend the use of purified C48-6 mAb in our special formulation for immunohistochemistry, Cat. no. 550340.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557275 Rev. 5
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The C48-6 antibody is specific for keyhole limpet hemocyanin (KLH).

557275 Rev. 5
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
557275 Rev.5
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557275 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.