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准备和存储
The PE conjugate is supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Anti–P-glycoprotein (P-gp), clone 15D3, is generated from the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a BA3T3 fibroblast line (BATV.2) transfected with the human MDR1 gene. Anti–P-glycoprotein (P-gp) recognizes a 170-kdalton (kDa) protein from multidrug resistant (MDR) cells.
研发参考 (10)
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Beck WT, Grogan TM, Willman CL, et al. Methods to detect P-glycoprotein–associated multidrug resistance in patients tumors: concensus recommendations. Cancer Res. 1996; 56:3010-3020. (Biology).
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Chan HS, Haddad G, Zheng L, Bradley G, Dalton WS, Ling V. Sensitive immunofluorescence detection of the expression of P-glycoprotein in malignant cells.. Cytometry. 1997; 29(1):65-75. (Biology). 查看参考
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Dalton WS, Durie BG, Alberts DS, Gerlach JH, Cress AE. Characterization of a new drug-resistant human myeloma cell line that expresses P-glycoprotein.. Cancer Res. 1986; 46(10):5125-30. (Biology). 查看参考
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Drach D, Zhao S, Drach J, et al. Subpopulations of normal peripheral blood and bone marrow cells express a functional multidrug resistant phenotype.. Blood. 1992; 80(11):2729-34. (Biology). 查看参考
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Filipits M, Suchomel RW, Dekan G, et al. MRP and MDR1 gene expression in primary breast carcinomas.. Clin Cancer Res. 1996; 2(7):1231-7. (Biology). 查看参考
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Gupta S, Aggarwal S. P-glycoprotein in cells of the human immune system. The Immunologist 4/3. 1996; 86-90. (Biology).
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Klimecki WT, Futscher BW, Grogan TM, Dalton WS. P-glycoprotein expression and function in circulating blood cells from normal volunteers.. Blood. 1994; 83(9):2451-8. (Biology). 查看参考
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List AF. Role of multidrug resistance and its pharmacological modulation in acute myeloid leukemia.. Leukemia. 1996; 10(6):937-42. (Biology). 查看参考
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Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
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