PE Mouse Anti-Human CD54

BD™ PE Mouse Anti-Human CD54

Name: PE Mouse Anti-Human CD54
Brand: BD™
SKU: 347977

克隆 LB-2

(RUO (GMP))

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商品详情

Brand: BD™

Alternative Name: Human ICAM-1, HICAM-1; BB2; P3.58

Reactivity: Human

Isotype: Mouse IgG2b, κ

Immunogen: Major histocompatibility Class II antigen negative variant of human B-lymphoblastoid cell line

Application: Flow cytometry

Concentration: 3 μg/mL

Vol. Per Test: 20 μL

Workshop Number: IV B50, M165

Entrez Gene ID: 3383

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

准备和存储

The monoclonal antibody is supplied as 6 µg purified immunoglobulin in 2.0 mL (3 µg/mL) of phosphate-buffered saline. The PE conjugate is supplied as 6 µg in 2.0 mL (3 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347977 Rev. 1

抗体详情

LB-2

CD54, clone LB-2, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II–negative human B-lymphoblastoid cell line 6.16c13.

CD54 (Leu-54) recognizes the intercellular adhesion molecule (ICAM-1), Mr 90 kdaltons (kDa). The CD54 antigen is a ligand for the leucocyte function–associated antigen-1 (CD11a/CD18 [LFA-1]) and influences both LFA-1–dependent adhesion of leucocytes to endothelial cells and immune functions involving cell-to-cell contact.

347977 Rev. 1

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

347977 Rev.1

报价单和参考

View product citations for antibody "347977" on CiteAb

研发参考 (10)

Bangham CR, McMichael AJ. Nosing ahead in the cold war. Nature. 1990; 344:16. (Biology).
Boyd AW, Dunn SM, Fecondo JV, et al. Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989; 73:1896-1903. (Biology).
Clark EA, Ledbetter JA, Holly RC, Dinndorf PA, Shu G. Polypeptides on human B lymphocytes associated with cell activation. Hum Immunol. 1986; 16(1):100-113. (Biology). 查看参考
Dustin ML, Staunton DE, Springer TA. Supergene families meet in the immune system. Immunol Today. 1988; 9:213-215. (Biology).
Johnson JP, Shaw S. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:681-683.
Makgoba MW, Sanders ME, Ginther Luce GE, et al. ICAM-1: a ligand for LFA-1–dependent adhesion of B, T, and myeloid cells. Nature. 1988; 331:86-88. (Biology).
Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber W, Merluzzi VJ. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Nature. 1990; 344:70-72. (Biology).
Patarroyo M, Clark EA, Prieto J, Kantor C, Gahmberg CG. Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB-2. FEBS Lett. 1987 January; 210(2):127-131. (Biology). 查看参考
Patarroyo M, Makgoba MW. Leucocyte adhesion to cells. Molecular basis, physiological relevance, and abnormalities.. Scand J Immunol. 1989; 30(2):129-64. (Biology). 查看参考
Staunton DE, Dustin ML, Springer TA. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Nature. 1989; 339:61-64. (Biology).

查看所有文件 (10)

347977 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.