PE Mouse Anti-Human CD16

BD™ PE Mouse Anti-Human CD16

Name: PE Mouse Anti-Human CD16
Brand: BD™
SKU: 347617

克隆 B73.1

(RUO (GMP))

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商品详情

Brand: BD™

Alternative Name: IgG Fc receptor III; IGFR3; FCG3; FCGR3; FCGRIII; FcγRIII

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human NK Cells

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: IV NL402

Entrez Gene ID: 916

RRID: AB_400331

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

准备和存储

The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate -buffered saline (PBS). The FITC conjugate is supplied as 250 μg in 2.0 mL (125 μg/mL) of PBS. The PE conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 50 μg in 0.5 mL (100 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347617 Rev. 1

抗体详情

B73.1

CD16 (Leu-11a), clone NKP15, is derived from hybridization of mouse P3-X63-Ag8 myeloma cells with spleen cells from BALB/c mice immunized with large granular lymphocytes.

CD16 (Leu-11b), clone GO22, is derived from hybridization of mouse P3-X63 myeloma cells with spleen cells from BALB/c mice immunized with granulocytes.

CD16 (Leu-11c), clone B73.1, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with NK lymphocytes.

CD16 antibodies recognize an Mr 50- to 65-kilodalton (kd) antigen present on human natural killer (NK) lymphocytes that is the IgG Fc receptor III.

347617 Rev. 1

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

347617 Rev.1

报价单和参考

研发参考 (13)

Campbell JJ, Qin S, Unutmaz D, et al. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol. 2001; 166(11):6477-6482. (Biology). 查看参考
Cooper MA, Fehniger TA, Caligiuri MA. The biology of human natural killer–cell subsets. Trends Immunol. 2001; 22(11):633-640. (Biology). 查看参考
Galandrini R, Tassi I, Mattia G, et al. SH2-containing inositol phosphatase (SHIP-1) transiently translocates to raft domains and modulates CD16-mediated cytotoxicity in human NK cells. Blood. 2001; 100(13):4581-4589. (Biology). 查看参考
Gerosa F, Baldani-Guerra B, Nisii C, Marchesini V, Carra G, Trinchieri G. Reciprocal activating interaction between natural killer cells and dendritic cells. J Exp Med. 2002; 195(3):327-333. (Biology). 查看参考
Lanier LL, Kipps TJ, Phillips JH. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med. 1985; 162(6):2089-2106. (Biology). 查看参考
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). 查看参考
Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 查看参考
Perussia B, Acuto O, Terhorst C, et al. Human natural killer cells analyzed by B73-1, a monoclonal antibody blocking Fc receptor functions, II: studies of B73-1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983; 130:2142-2148. (Biology).
Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983; 130(5):2133-2141. (Biology). 查看参考
Perussia B, Trinchieri G, Jackson A, et al. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984; 133(1):180-189. (Biology). 查看参考
Phillips JH, Babcock GF. A monoclonal antibody reactive against purified human natural killer cells and granuocytes. Immunol Letters. 1983; 6:143. (Biology).
Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:517-542.
Thompson J, Goeken N, Brown S, Rhodes J. Phenotypic definition of human monocyte/macrophage subpopulations by monoclonal antibodies, II: Distinction of non-T, non-B natural killer cells (NK) from antigen-presenting stimulating cells in the mixed lymphocyte response (MLR) Presented at the American Association for Clinical Histocompatibility Testing. (Biology).

查看所有文件 (13)

347617 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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