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      PE Mouse Anti-Human CD154
      商品详情
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      BD™
      CD40LG; CD40 ligand; CD40L; gp39; TNFSF5; T-BAM; TRAP
      Human
      Mouse BALB/c IgG1, κ
      sgp39-CD8 and Activated Human PBL
      Flow cytometry
      12.5 μg/mL
      20 μL
      959
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      准备和存储

      Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340477 Rev. 1
      抗体详情
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      89-76

      CD154, clone 89-76, is derived from hybridization of mouse NS-1 cells with spleen cells from BALB/c mice immunized with sgp39-CD8 and activated human peripheral blood lymphocytes (PBLs).

      CD154 (CD40 ligand or CD40L) recognizes a 39-kilodalton (kDa) antigen that is a member of the tumor necrosis factor (TNF) receptor family. CD154 is transiently expressed on activated CD4 T lymphocytes. The expression of CD154 by activated T-helper cells triggers B-cell cycling through binding to CD40. This co-receptor interaction is required for B-lymphocyte maturation, response to T-dependent ligands, and isotype switching. T-lymphocyte proliferation and generation of cytotoxic T lymphocytes (CTLs) is associated with the presence of CD154. Defects in the CD154 gene are associated with the X-linked hyper IgM syndrome.

      340477 Rev. 1
      格式详情
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340477 Rev.1
      报价单和参考
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      研发参考 (23)

      1. Armitage RJ, Fanslow WC, Strockbine L, et al. Molecular and biological characterization of a murine ligand for CD40.. Nature. 1992; 357(6373):80-2. (Biology). 查看参考
      2. Aruffo A, Farrington M, Hollenbaugh D, et al. The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell. 1993; 72(2):291-300. (Biology). 查看参考
      3. Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). 查看参考
      4. Axcrona K, Gray D, Leanderson T. Regulation of B cell growth and differentiation via CD21 and CD40. Eur J Immunol. 1996; 26(9):2203-2207. (Biology). 查看参考
      5. Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). 查看参考
      6. Carbone A, Gloghini A, Gruss HJ, Pinto A. CD40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin's disease.. Am J Pathol. 1995; 147(4):912-22. (Biology). 查看参考
      7. Cayabyab M, Phillips JH, Lanier LL. CD40 preferentially costimulates activation of CD4+ T lymphocytes.. J Immunol. 1994; 152(4):1523-31. (Biology). 查看参考
      8. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      9. Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). 查看参考
      10. Clinical and Laboratory Standards Institute. 2005. (Biology).
      11. Ding L, Green JM, Thompson CB, Shevach EM. B7/CD28-dependent and -independent induction of CD40 ligand expression.. J Immunol. 1995; 155(11):5124-32. (Biology). 查看参考
      12. Durie FH, Foy TM, Masters SR, Laman JD, Noelle RJ. The role of CD40 in the regulation of humoral and cell-mediated immunity.. Immunol Today. 1994; 15(9):406-11. (Biology). 查看参考
      13. Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). 查看参考
      14. Fuleihan R, Ahern D, Geha RS. CD40 ligand expression is developmentally regulated in human thymocytes.. Clin Immunol Immunopathol. 1995; 76(1 Pt 1):52-8. (Biology). 查看参考
      15. Gauchat JF, Aubry JP, Disanto J, et al. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:416-418.
      16. Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.. J Immunol. 1995; 155(2):556-67. (Biology). 查看参考
      17. Hollenbaugh D, Grosmaire LS, Kullas CD, et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. EMBO J. 1992; 11(12):4313-21. (Biology). 查看参考
      18. Lane P, Traunecker A, Hubele S, Inui S, Lanzavecchia A, Gray D. Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.. Eur J Immunol. 1992; 22(10):2573-8. (Biology). 查看参考
      19. Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992; 89(14):6550-6554. (Biology). 查看参考
      20. Noelle RJ. The role of gp39 (CD40L) in immunity.. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S203-7. (Biology). 查看参考
      21. Ramesh N, Morio T, Fuleihan R, et al. CD40-CD40 ligand (CD40L) interactions and X-linked hyperIgM syndrome (HIGMX-1).. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S208-13. (Biology). 查看参考
      22. Roy M, Waldschmidt T, Aruffo A, Ledbetter JA, Noelle RJ. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J Immunol. 1993; 151(5):2497-2510. (Biology). 查看参考
      23. Spriggs MK, Armitage RJ, Strockbine L, et al. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. J Exp Med. 1992; 176(6):1543-50. (Biology). 查看参考
      查看所有文件 (23) 查看更少内容
      340477 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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