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PE-Cy™7 Mouse Anti-Human CD45RA
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Mouse BALB/c IgG1, κ
Low-buoyant–density human lymphocytes
Flow cytometry
50 μg/mL
5 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.


The monoclonal antibody is supplied as 50 μg of purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate buffered saline (PBS). The FITC conjugate is supplied as 100 μg in 2.0 mL (50 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

337167 Rev. 1
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The CD45RA antibody, clone L48, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with low-buoyant–density human lymphocytes.

The CD45RA antibody recognizes a 220-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens.

337167 Rev. 1
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PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
781 nm
337167 Rev.1
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研发参考 (11)

  1. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  2. Gamadia LE, Rentenaar RJ, Baars PA, et al. Differentiation of cytomegalovirus-specific CD8(+) T cells in healthy and immunosuppressed virus carriers.. Blood. 2001; 98(3):754-61. (Biology). 查看参考
  3. Ledbetter JA, Rose LM, Spooner CE, Beatty PG, Martin PJ, Clark EA. Antibodies to common leukocyte antigen p220 influence human T cell proliferation by modifying IL 2 receptor expression.. J Immunol. 1985; 135(3):1819-25. (Biology). 查看参考
  4. Mackus WJ, Frakking FN, Grummels A, et al. Expansion of CMV-specific CD8+CD45RA+CD27- T cells in B-cell chronic lymphocytic leukemia.. Blood. 2003; 102(3):1057-63. (Biology). 查看参考
  5. Maldonado A, Mueller YM, Thomas P, Bojczuk P, O'Connors C, Katsikis PD. Decreased effector memory CD45RA+ CD62L- CD8+ T cells and increased central memory CD45RA- CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients.. Arthritis Res Ther. 2003; 5(2):R91-6. (Biology). 查看参考
  6. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF. The isolation and characterization of the human suppressor/inducer T-cell subset. J Immunol. 1985; 134:1508-1515. (Biology).
  7. Plaeger-Marshall S, Hultin P, Bertolli J, et al. Activation and differentiation antigens on T cells of healthy, at-risk, and HIV-infected children.. J Acquir Immune Defic Syndr. 1993; 6(9):984-93. (Biology). 查看参考
  8. Rose LM, Ginsberg AH, Rothstein TL, Ledbetter JA, Clark EA. Selective loss of a subset of T helper cells in active multiple sclerosis.. Proc Natl Acad Sci USA. 1985; 82(21):7389-93. (Biology). 查看参考
  9. Serra HM, Krowka JF, Ledbetter JA, Pilarski LM. Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event.. J Immunol. 1988; 140(5):1435-41. (Biology). 查看参考
  10. Sobel RA, Hafler DA, Castro EE, Morimoto C, Weiner HL. The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions.. J Immunol. 1988; 140(7):2210-4. (Biology). 查看参考
  11. Walker EB, Haley D, Miller W, et al. gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells.. Clin Cancer Res. 2004; 10(2):668-80. (Biology). 查看参考
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337167 Rev. 1

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