FITC Mouse Anti-Human CD2

BD™ FITC Mouse Anti-Human CD2

Name: FITC Mouse Anti-Human CD2
Brand: BD™
SKU: 347593

克隆 S5.2

(RUO (GMP))

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科学资源

商品详情

Brand: BD™

Alternative Name: LFA-2; Lymphocyte function antigen-2; Rosette receptor; T11/Leu-5; Leu-5b

Reactivity: Human

Isotype: Mouse IgG2a

Immunogen: Alloantigen-activated T Cells

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Workshop Number: II T16

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

准备和存储

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347593 Rev. 1

抗体详情

S5.2

The CD2 antibody, clone S5.2, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with T lymphocytes activated by mixed lymphocyte culture.

The CD2 antibody recognizes a human lymphocyte antigen, 45 to 50 kilodaltons (kDa), which also forms the binding site for sheep erythrocytes.

347593 Rev. 1

格式详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: FITC

激发源: Blue 488 nm

最多激发源: 494 nm

最多发射源: 518 nm

347593 Rev.1

报价单和参考

研发参考 (9)

Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin.. Transplantation. 1981; 31(4):283-9. (Biology). 查看参考
Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T-cell activation via the physiological ligands for CD2 and the T-cell receptor. J Exp Med. 1988; 168:1145-1156. (Biology).
Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells.. J Immunol. 1999; 163(11):5920-8. (Biology). 查看参考
Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.. J Immunol. 1981; 126(6):2117-22. (Biology). 查看参考
Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen.. Proc Natl Acad Sci USA. 1990; 87(7):2603-7. (Biology). 查看参考
MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). 查看参考
Moingeon P, Chang H, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T-lymphocyte antigen-recognition function. Nature. 1989; 339:312-314. (Biology).

查看所有文件 (9)

347593 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.